Mouse CPE Gene cDNA Clone / ORF Clone

CPE cDNA Clone Datasheet

CPE cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013494.3
• cDNA Size: 1431
• Gene Synonym: CPH; fat; Cph1; Cph-1; R74677; MGC7101; Cpe
• Source: Mouse

CPE cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Carboxypeptidase E DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 779 A/T resulting in the amino acid glu substitution by val and 330 T/C,345 T/C, 367 C/T,372 T/C not causing the amino acid variation.

CPE cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CPE cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CPE cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGGGC GCGGAGGACG GGTGCTGCTG GCGCTGTGTG CCGCGCTGGT GGCCGGCGGG
61       TGGCTGCTGA CGGCTGAAGC CCAGGAGCCC GGGGCGCCAG CGGCTGGCAT GAGGCGCCGC
121      CGGCGGCTCC AGCAAGAGGA CGGCATCTCC TTCGAGTACC ACCGCTATCC AGAGCTGCGC
181      GAGGCGCTGG TGTCCGTATG GCTGCAGTGC ACCGCCATCA GCAGAATCTA CACAGTGGGG
241      CGCAGCTTCG AGGGCCGGGA GCTCCTGGTC ATCGAGCTGT CTGACAACCC CGGGGTCCAT
301      GAGCCGGGTG AACCTGAATT TAAATACATC GGGAACATGC ATGGCAATGA GGCGGTTGGA
361      CGGGAATTGC TCATTTTCTT GGCCCAGTAC CTGTGTAACG AGTACCAGAA AGGCAATGAG
421      ACAATTGTCA ACCTGATCCA CAGCACCCGA ATTCATATCA TGCCCTCCTT GAACCCCGAC
481      GGCTTTGAGA AAGCCGCATC GCAGCCCGGC GAGCTGAAGG ACTGGTTTGT GGGCCGCAGC
541      AACGCCCAGG GAATAGATCT GAACCGTAAC TTCCCAGACC TGGACAGGAT CGTGTATGTT
601      AATGAGAAAG AAGGCGGTCC TAACAATCAC CTGCTGAAGA ATCTGAAGAA AATTGTGGAC
661      CAAAATTCAA AGCTTGCCCC CGAGACCAAG GCTGTCATTC ACTGGATCAT GGACATTCCA
721      TTTGTGCTTT CTGCCAATCT GCACGGAGGA GACCTTGTGG CTAATTACCC ATATGATGTG
781      ACACGGAGCG GTACTGCTCA CGAATACAGT TCCTGCCCTG ATGACGCAAT TTTCCAAAGC
841      TTGGCTCGCG CGTACTCTTC TTTCAACCCA GTCATGTCTG ACCCCAATCG ACCTCCCTGT
901      CGCAAGAATG ACGATGACAG CAGCTTTGTA GATGGAACGA CCAATGGTGG TGCATGGTAC
961      AGCGTCCCCG GTGGAATGCA AGACTTCAAT TACCTGAGCA GCAACTGCTT CGAGATCACT
1021     GTGGAGCTTA GCTGTGAGAA GTTCCCACCG GAAGAGACTC TCAAAAGCTA CTGGGAAGAT
1081     AACAAAAACT CCCTCATCAG CTACCTGGAG CAGATACACC GAGGTGTTAA AGGGTTTGTC
1141     CGTGACCTTC AGGGTAACCC GATTGCCAAC GCAACCATCT CTGTGGACGG GATAGACCAT
1201     GATGTCACCT CGGCTAAGGA TGGGGATTAC TGGCGATTGC TTGCTCCTGG AAACTATAAA
1261     CTTACAGCCT CCGCTCCTGG CTACCTGGCA ATCACAAAGA AAGTGGCAGT TCCTTTTAGC
1321     CCTGCTGTTG GGGTGGACTT TGAGCTTGAG TCTTTCTCTG AAAGGAAGGA GGAGGAGAAG
1381     GAAGAATTGA TGGAGTGGTG GAAAATGATG TCAGAAACTT TGAATTTTTA A

 

Quality Control:cDNA sequencing results is available upon request.