Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human General Information
Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human
Solid Phase Sandwich ELISA
Quantitative determination of Human Carboxypeptidase B1
1. Capture Antibody 0.2 mg/mL of mouse anti-CPB1 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10501-MM06)
2. Detection Antibody 0.5 mg/mL of rabbit anti-CPB1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10501-R108)
3. Standard Each vial contains 30 ng of recombinant CPB1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
This Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Carboxypeptidase B1 . It contains Human Carboxypeptidase B1 capture antibody, Human Carboxypeptidase B1 detector antibody
and a highly purified
recombinant Human Carboxypeptidase B1 protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human Images
Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human Alternative Names
CPB Matched ELISA Antibody Pair Set, Human;PASP Matched ELISA Antibody Pair Set, Human;PCPB Matched ELISA Antibody Pair Set, Human
Carboxypeptidase B1 Background Information
Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
carboxypeptidase B1 (tissue)
Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581. Pezzilli, R. et al., 1994, Digestion. 55: 73-77. Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.