Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human

Cat: SEK10501

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Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human General Information

Product name
Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human Carboxypeptidase B1
Assay range
6.25-400
Materials provided
1. Capture Antibody 0.2 mg/mL of mouse anti-CPB1 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10501-MM06)
2. Detection Antibody 0.5 mg/mL of rabbit anti-CPB1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10501-R108)
3. Standard Each vial contains 30 ng of recombinant CPB1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
Product overview
This Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Carboxypeptidase B1 . It contains Human Carboxypeptidase B1 capture antibody, Human Carboxypeptidase B1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Carboxypeptidase B1 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human Images

Human Carboxypeptidase B1/CPB1 ELISA Pair Set Standard Curve 13845

Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human Alternative Names

CPB Matched ELISA Antibody Pair Set, Human;PASP Matched ELISA Antibody Pair Set, Human;PCPB Matched ELISA Antibody Pair Set, Human

Carboxypeptidase B1 Background Information

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Full Name
carboxypeptidase B1 (tissue)
References
  • Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581.
  • Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
  • Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Mouse 12.5 pg/ml SEK50386
    Carboxypeptidase B1 ELISA Kit, Human Serum KIT10501
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