Carboxypeptidase B1 / CPB1 Antibody

Carboxypeptidase B1 / CPB1 Antibody Datasheet

Carboxypeptidase B1 / CPB1 Antibody Product Information

• Immunogen: Recombinant Mouse Carboxypeptidase B1 / CPB1 protein (Catalog#50386-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 117
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carboxypeptidase B1 / CPB1 (rM Carboxypeptidase B1 / CPB1; Catalog#50386-M08H; NP_083982.1; Met 1-Tyr 415).

Carboxypeptidase B1 / CPB1 Antibody Usage Guide

• Specificity: Mouse Carboxypeptidase B1 / CPB1
• No cross-reactivity in ELISA with

  Human CPB1

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse CPB1 in WB. Using a DAB detection system, the detection limit for Mouse CPB1 is approximately 0.25 ng/lane under non-reducing conditions and 0.125 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CPB1. The detection limit for Mouse CPB1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Carboxypeptidase B1 / CPB1 Antibody Background

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

References

1. Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581.
2. Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
3. Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.