Anti-Carboxypeptidase B1 Antibody

Cat: 10501-MM06
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All Carboxypeptidase B1 Reagents
CRO Services
Product recommended

All Carboxypeptidase B1 Reagents
Anti-Carboxypeptidase B1 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-Carboxypeptidase B1 Antibody
Validated applications
ELISA,ELISA(Cap)
Species reactivity
Reacts with: Human
Specificity
Human Carboxypeptidase B1
No cross-reactivity in ELISA with Mouse CPB1, Human CPA1, Human CPA2, Human CPE
Immunogen
Recombinant Human CPB1 protein (Catalog#10501-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CPB1 (rh CPB1; Catalog#10501-H08H; NP_001862.2; Met 1-Tyr 417). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .
Source
Monoclonal Mouse IgG1 Clone #2C10C6B12
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carboxypeptidase B1 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ELISA(Cap) 1:250-1:2000 This antibody will detect Human Carboxypeptidase B1 / CPB1 in ELISA pair set (Catalog: # SEK10501). In a sandwich ELISA, it can be used as capture antibody when paired with (Catalog: # 10501-R108).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carboxypeptidase B1 Antibody Alternative Names
Anti-CPB Antibody;Anti-PASP Antibody;Anti-PCPB Antibody
Carboxypeptidase B1 Background Information

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Full Name
carboxypeptidase B1 (tissue)
References
  • Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581.
  • Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
  • Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Carboxypeptidase B1 Antibody Rabbit Monoclonal ELISA(Cap) 50386-R001
    Anti-Carboxypeptidase B1 Antibody Rabbit Polyclonal ELISA 50386-RP01
    Anti-Carboxypeptidase B1 Antibody Rabbit Monoclonal ELISA(Det) 50386-R008
    Anti-Carboxypeptidase B1 Antibody Rabbit Monoclonal ELISA 50386-R117
    Anti-Carboxypeptidase B1 Antibody Rabbit Polyclonal ELISA 50386-RP02
    Anti-Carboxypeptidase B1 Antibody Rabbit Polyclonal ELISA 10501-RP01
    Anti-Carboxypeptidase B1 Antibody Rabbit Monoclonal ELISA,IHC-P 10501-R329
    Anti-Carboxypeptidase B1 Antibody Mouse Monoclonal IHC-P 10501-MM07
    Anti-Carboxypeptidase B1 Antibody Rabbit Polyclonal ELISA 10501-RP02
    Anti-Carboxypeptidase B1 Antibody Rabbit Monoclonal ELISA(Det) 10501-R108
    info info
    添加购物车成功! 添加购物车失败!