Carboxypeptidase A2 Matched ELISA Antibody Pair Set,Human

Cat: SEK10499
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Carboxypeptidase A2 Matched ELISA Antibody Pair Set,Human General Information
Product name
Carboxypeptidase A2 Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human Carboxypeptidase A2
Assay range
12.5-800
Materials provided
1. Capture Antibody 1.0 mg/mL of mouse anti-CPA2 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10499-MM02)
2. Detection Antibody 0.5 mg/mL rabbit anti-CPA2 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10499-R009)
3. Standard Each vial contains 50 ng of recombinant CPA2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 800 pg/mL is recommended.
Product overview
This Carboxypeptidase A2 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Carboxypeptidase A2 . It contains Human Carboxypeptidase A2 capture antibody, Human Carboxypeptidase A2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Carboxypeptidase A2 protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Carboxypeptidase A2 Matched ELISA Antibody Pair Set,Human Images
Human Carboxypeptidase A2/CPA2 ELISA Pair Set Standard Curve 13844
Carboxypeptidase A2 Matched ELISA Antibody Pair Set,Human Alternative Names
CPA2 Matched ELISA Antibody Pair Set, Human
Carboxypeptidase A2 Background Information

Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.

Full Name
carboxypeptidase A2
References
  • Catasus, L. et al., 1995. J. Biol. Chem. 270: 6651-6657.
  • Aloy, P. et al., 1998, Biol. Chem. 379: 149-155.
  • Laethem, RM. et al., 1996, Arch. Biochem. Biophys.332: 8-18.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    info info
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