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Carboxypeptidase A2 / CPA2 Antibody, Mouse MAb

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Human CPA2 Antibody Product Information
Immunogen:Recombinant Human CPA2 protein (Catalog#10499-H08H)
Clone ID:1G7G10E4
Ig Type:Mouse IgG2a
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CPA2 (rh CPA2; Catalog#10499-H08H; NP_001859.1; Met 1-Tyr 417). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CPA2 Antibody Usage Guide
Specificity:Human CPA2 / Carboxypeptidase A2
No cross-reactivity in WB and ELISA with
Human CPE / Carboxypeptidase E
Human Carboxypeptidase B1 / CPB1 / PCPB
Human cell lysate (293 cell line)
Application:ELISA, IHC-P

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CPA2. The detection limit for Human CPA2 is 0.039 ng/well.

IHC-P: 10-30 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CPA2 Antibody IHC Application Image
Carboxypeptidase A2 / CPA2 Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Immunochemical staining of human CPA2 in human pancreas (from two donors) with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to pancreatic acinus.
Other CPA2 Antibody Products
Carboxypeptidase A2/CPA2 Background

Human Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.

Human Carboxypeptidase A2/CPA2 References
  • Catasus, L. et al., 1995. J. Biol. Chem. 270: 6651-6657.
  • Aloy, P. et al., 1998, Biol. Chem. 379: 149-155.
  • Laethem, RM. et al., 1996, Arch. Biochem. Biophys.332: 8-18.
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    Catalog: 10499-MM07-200
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