|IP||4-6 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CPA1 was immunoprecipitated using:
Lane A:0.5 mg Jurkat Whole Cell Lysate2 µL anti-CPA1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CPA1 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:
Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.
Performed under reducing conditions.Predicted band size: 50 kDa
Observed band size: 50 kDa
Anti-CPA1 rabbit polyclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell LysateLysates/proteins at 60 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:55 kDa
Observed band size:47 kDa
(We are unsure as to the identity of these extra bands.)
Carboxypeptidase A1 （CPA1）is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Carboxypeptidase A Antibody||Rabbit||Polyclonal||ELISA||50448-RP02|
|Anti-Carboxypeptidase A Antibody||Rabbit||Monoclonal||ELISA||50448-R003|
|Anti-Carboxypeptidase A Antibody||Rabbit||Polyclonal||ELISA||50448-RP01|
|Anti-Carboxypeptidase A Antibody||Rabbit||Monoclonal||ELISA||50448-R001|
|Anti-Carboxypeptidase A Antibody||Rabbit||Polyclonal||ELISA||10504-RP01|
|Anti-Carboxypeptidase A Antibody||Mouse||Monoclonal||IHC-P||10504-MM03|