Anti-Carboxypeptidase A Antibody

Cat: 10504-RP02

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Anti-Carboxypeptidase A Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Carboxypeptidase A Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Carboxypeptidase A
Recombinant Human Carboxypeptidase A1 / CPA1 protein (Catalog#10504-H08H)
Produced in rabbits immunized with purified, recombinant Human Carboxypeptidase A1 / CPA1 (rh Carboxypeptidase A1 / CPA1; Catalog#10504-H08H; NP_001859.1; Met 1-Tyr 419). Carboxypeptidase A1 / CPA1 specific IgG was purified by Human Carboxypeptidase A1 / CPA1 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carboxypeptidase A Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IHC-P 1:2500-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carboxypeptidase A Antibody (Rabbit Polyclonal antibody) Images
Human Carboxypeptidase A1/CPA1 Immunohistochemistry(IHC) 4372
Immunochemical staining of human CPA1 in human pancreas with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Human Carboxypeptidase A1/CPA1 Immunoprecipitation(IP) 7997

CPA1 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

2 µL anti-CPA1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CPA1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 50 kDa

Observed band size: 50 kDa

Human Carboxypeptidase A1/CPA1 Western blot (WB) 13242

Anti-CPA1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 60 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:47 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Carboxypeptidase A Antibody Alternative Names
Anti-CPA Antibody
Carboxypeptidase A Background Information

Carboxypeptidase A1 (CPA1)is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of  residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated.

Full Name
carboxypeptidase A1 (pancreatic)
  • Catasus, L. et al., 1992, Biochem. J. 287: 299-303.
  • Moulard, M. et al., 1990, FEBS. Lett. 261: 179-183.
  • Aloy, P. et al., 1998, Biol. Chem. 379: 149-155.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Carboxypeptidase A Antibody Rabbit Polyclonal ELISA 50448-RP02
    Anti-Carboxypeptidase A Antibody Rabbit Monoclonal ELISA 50448-R003
    Anti-Carboxypeptidase A Antibody Rabbit Polyclonal ELISA 50448-RP01
    Anti-Carboxypeptidase A Antibody Rabbit Monoclonal ELISA 50448-R001
    Anti-Carboxypeptidase A Antibody Rabbit Polyclonal ELISA 10504-RP01
    Anti-Carboxypeptidase A Antibody Mouse Monoclonal IHC-P 10504-MM03
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