Carboxypeptidase A1 / CPA1 Antibody

Carboxypeptidase A1 / CPA1 Antibody Datasheet

Carboxypeptidase A1 / CPA1 Antibody Product Information

• Immunogen: Recombinant Mouse Carboxypeptidase A1 / CPA1 protein (Catalog#50448-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 001
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carboxypeptidase A1 / CPA1 (rM Carboxypeptidase A1 / CPA1; Catalog#50448-M08H; NP_079626.2; Met 1-Tyr 419).

Carboxypeptidase A1 / CPA1 Antibody Usage Guide

• Specificity: Mouse Carboxypeptidase A1 / CPA1
• Has cross-reactivity in ELISA with
  

  Human CPA1

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CPA1. The detection limit for Mouse CPA1 is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Carboxypeptidase A1 / CPA1 Antibody Background

Carboxypeptidase A1 (CPA1) is secreted as a pancreatic procarboxypeptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition. Three different forms of pancreatic procarboxypeptidase A have been isolated.

References

1. Catasus, L. et al., 1992, Biochem. J. 287: 299-303.
2. Moulard, M. et al., 1990, FEBS. Lett. 261: 179-183.
3. Aloy, P. et al., 1998, Biol. Chem. 379: 149-155.