Anti-Carbonic Anhydrase XIV Antibody

Cat: 10458-R004
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All Carbonic Anhydrase XIV Reagents
CRO Services
Product recommended

All Carbonic Anhydrase XIV Reagents
Anti-Carbonic Anhydrase XIV Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Carbonic Anhydrase XIV Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human Carbonic Anhydrase XIV
No cross-reactivity in ELISA with Human CAIX / CA9 Human CA10 / CARPX Human CA12 / CAXII Mouse CA14 / Car14
Immunogen
Recombinant Human CA14 protein (Catalog#10458-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CA14 / Car14 extracellular domain (rh CA14; Catalog#10458-H08H; NP_036245.1; Met 1-Met 290).
Source
Monoclonal Rabbit IgG Clone #004
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carbonic Anhydrase XIV Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carbonic Anhydrase XIV Antibody (Rabbit Monoclonal antibody) Images
Human Carbonic Anhydrase XIV Western blot (WB) 16046

Anti-CA14 rabbit monoclonal antibody at 1:500 dilution

Lane A: Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:38 kDa

Observed band size:34 kDa

Anti-Carbonic Anhydrase XIV Antibody Alternative Names
Anti-CA14 Antibody;Anti-CAXiV Antibody
Carbonic Anhydrase XIV Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.

Full Name
carbonic anhydrase XIV
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Parkkila S, et al. (2001) Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. PNAS. 98(4): 1918-23.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Carbonic Anhydrase XIV Antibody Rabbit Polyclonal WB,ELISA 50013-RP01
    Anti-Carbonic Anhydrase XIV Antibody Rabbit Polyclonal WB,ELISA 50013-RP02
    Anti-Carbonic Anhydrase XIV Antibody Rabbit Monoclonal ELISA 50013-R033
    Anti-Carbonic Anhydrase XIV Antibody Rabbit Monoclonal ELISA(Cap) 10458-R110
    Anti-Carbonic Anhydrase XIV Antibody Rabbit Monoclonal ELISA,IHC-P,ICC/IF,IF 10458-R515
    Anti-Carbonic Anhydrase XIV Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 10458-T60
    Anti-Carbonic Anhydrase XIV Antibody Rabbit Polyclonal ELISA 10458-RP01
    info info
    添加购物车成功! 添加购物车失败!