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Carbonic Anhydrase XIV / CA14 Antibody, Rabbit PAb, Antigen Affinity Purified

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Human CA14 Antibody Product Information
Immunogen:Recombinant Human CA14 protein (Catalog#10458-H08H)
Clone ID:
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human CA14 / Car14 extracellular domain (rh CA14; Catalog#10458-H08H; NP_036245.1; Met 1-Met 290). CA14 specific IgG was purified by human CA14 affinity chromatography.
Human CA14 Antibody Usage Guide
Specificity:Human CA14 / Car14
Application:ELISA, IHC-P

ELISA: 0.5-1.0 μg/mL

This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human CA14. The detection limit for Human CA14 is 0.0049 ng/well.

IHC-P: 0.2-2 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CA14 Antibody IHC Application Image
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Immunohistochemical staining of formalin fixed, paraffin-embedded human brain showing neuron staining (0.5 μg/mL).
Carbonic Anhydrase XIV / CA14 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Immunochemical staining of human CA14 in human liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of hepatocyte. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Carbonic Anhydrase XIV / CA14 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Other CA14 Antibody Products
Carbonic Anhydrase XIV Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.

Human Carbonic Anhydrase XIV References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Parkkila S, et al. (2001) Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. PNAS. 98(4): 1918-23.
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