CA12 Matched ELISA Antibody Pair Set,Human General Information
CA12 Matched ELISA Antibody Pair Set,Human
Solid Phase Sandwich ELISA
Quantitative determination of Human CA12
1. Capture Antibody 90 μg/mL of mouse anti-CA12 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10617-MM01)
2. Detection Antibody 0.25 mg/mL mouse anti-CA12 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10617-MM08)
3. Standard Each vial contains 37 ng of recombinant CA12. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.5 ng/mL is recommended.
This CA12 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human CA12 . It contains Human CA12 capture antibody, Human CA12 detector antibody
and a highly purified
recombinant Human CA12 protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
CA12 Matched ELISA Antibody Pair Set,Human Images
CA12 Matched ELISA Antibody Pair Set,Human Alternative Names
CA12 Matched ELISA Antibody Pair Set, Human;CAXII Matched ELISA Antibody Pair Set, Human;FLJ20151 Matched ELISA Antibody Pair Set, Human;HsT18816 Matched ELISA Antibody Pair Set, Human
CA12 Background Information
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.
carbonic anhydrase XII
Sahin, U. et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11810–11813. Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596 - 12601. Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 16903. Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262. Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602. Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.