Anti-CA12 Antibody

Cat: 10617-MM08
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Anti-CA12 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CA12 Antibody
Validated applications
ELISA,ELISA(Det)
Species reactivity
Reacts with: Human
Specificity
Human CA12
No cross-reactivity in ELISA with Mouse Carbonic Anhydrase XII / CA12 Human Carbonic Anhydrase II / CA2 Human Carbonic Anhydrase III / CA3 Human Carbonic Anhydrase IV / CA4 Human Carbonic Anhydrase VA / CA5A Human Carbonic Anhydrase VB / CA5B Human Carbonic Anhydrase VIII / CA8 Human Carbonic Anhydrase X / CA10 Human Carbonic Anhydrase XIII / CA13 Human Carbonic Anhydrase XIV / CA14 Human Carbonic Anhydrase IX / CA9 Human cell lysate (293 cell line)
Immunogen
Recombinant Human Carbonic Anhydrase XII / CA12 Protein (Catalog#10617-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase XII / CA12 (rh Carbonic Anhydrase XII / CA12; Catalog#10617-H08H; NP_001209.1; Met 1-Gln 291 B). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #2D6E1
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CA12 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human Carbonic Anhydrase XII in ELISA pair set (Catalog: # SEK10617). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10617-MM01).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CA12 Antibody Alternative Names
Anti-CA12 Antibody;Anti-CAXII Antibody;Anti-FLJ20151 Antibody;Anti-HsT18816 Antibody
CA12 Background Information

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I  membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

Full Name
carbonic anhydrase XII
References
  • Sahin, U. et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11810–11813.
  • Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596 - 12601.
  • Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 16903.
  • Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262.
  • Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602.
  • Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
  • Product Description Host Clonality Application Catalog# (PDF)
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    Anti-CA12 Antibody Rabbit Monoclonal ELISA 50014-R001
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    Anti-CA12 Antibody Mouse Monoclonal ELISA(Cap) 10617-MM01
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    Anti-CA12 Antibody (FITC) Mouse Monoclonal FCM 10617-MM11-F
    Anti-CA12 Antibody Mouse Monoclonal ICC/IF,IF 10617-MM04
    Anti-CA12 Antibody Rabbit Polyclonal WB,IHC-P 10617-RP02
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