Carbonic Anhydrase XII Antibody

Carbonic Anhydrase XII / CA12 Antibody Datasheet

Carbonic Anhydrase XII / CA12 Antibody Product Information

• Immunogen: Recombinant Mouse Carbonic Anhydrase XII / CA12 Protein (Catalog#50014-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 012
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Mouse Carbonic Anhydrase XII / CA12 ( rM Carbonic Anhydrase XII / CA12; Catalog#50014-M08H; NP_848483.2; Met 1 - Ser 301)

Carbonic Anhydrase XII / CA12 Antibody Usage Guide

• Specificity: Mouse Carbonic Anhydrase XII / CA12
• Immunochemistry: IHC-P: 1-10 μg/mL

  

  Immunochemical staining of mouse CA12 in mouse intestine with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of mouse CA12 in mouse kidney with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse CA12 in WB. Using a DAB detection system, the detection limit for Mouse CA12 is approximately 0.5 ng/lane under non-reducing conditions and 0.25 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CA12. The detection limit for Mouse CA12 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Carbonic Anhydrase XII / CA12 Antibody Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I  membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

References

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