Anti-CA12 Antibody

Cat: 10617-RP02
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Anti-CA12 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CA12 Antibody
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human CA12
Immunogen
Recombinant Human CA12 protein (Catalog#10617-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CA12 extracellular domain (rh CA12; Catalog#10617-H08H; NP_001209.1; Met 1-Gln 291). CA12 specific IgG was purified by human CA12 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CA12 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CA12 Antibody (Rabbit Polyclonal antibody) Images
Human Carbonic Anhydrase XII Immunohistochemistry(IHC) 4634
Immunochemical staining of human CA12 in human prostatic carcinomawith rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.
Human Carbonic Anhydrase XII Immunohistochemistry(IHC) 4635
Immunochemical staining of human CA12 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to nephric tubule.
Human Carbonic Anhydrase XII Immunohistochemistry(IHC) 4636
Immunochemical staining of human CA12 in human colon carcinomawith rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.
Human Carbonic Anhydrase XII Western blot (WB) 13255

Anti-CA12 rabbit polyclonal antibody at 1:500 dilution

Lane A: MCF7 Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:39 kDa

Observed band size:40 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CA12 Antibody Alternative Names
Anti-CA12 Antibody;Anti-CAXII Antibody;Anti-FLJ20151 Antibody;Anti-HsT18816 Antibody
CA12 Background Information

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I  membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.

Full Name
carbonic anhydrase XII
References
  • Sahin, U. et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11810–11813.
  • Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596 - 12601.
  • Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 16903.
  • Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262.
  • Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602.
  • Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CA12 Antibody Rabbit Monoclonal ELISA,IHC-P 50014-R012
    Anti-CA12 Antibody Rabbit Polyclonal ELISA 50014-RP01
    Anti-CA12 Antibody Rabbit Polyclonal ELISA,IHC-P 50014-RP02
    Anti-CA12 Antibody Rabbit Monoclonal ELISA 50014-R003
    Anti-CA12 Antibody Rabbit Monoclonal ELISA 50014-R001
    Anti-CA12 Antibody (PE) Mouse Monoclonal FCM 10617-MM07-P
    Anti-CA12 Antibody (PE) Mouse Monoclonal FCM 10617-MM11-P
    Anti-CA12 Antibody Mouse Monoclonal ICC/IF,IF 10617-MM04
    Anti-CA12 Antibody Mouse Monoclonal ELISA,ELISA(Det) 10617-MM08
    Anti-CA12 Antibody Mouse Monoclonal FCM,ICC/IF,IF 10617-MM07
    Anti-CA12 Antibody Mouse Monoclonal FCM 10617-MM11
    Anti-CA12 Antibody Mouse Monoclonal ELISA(Cap) 10617-MM01
    Anti-CA12 Antibody (FITC) Mouse Monoclonal FCM 10617-MM07-F
    Anti-CA12 Antibody (FITC) Mouse Monoclonal FCM 10617-MM11-F
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