|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CA8 was immunoprecipitated using:
Lane A:0.5 mg A549 Whole Cell Lysate2 µL anti-CA8 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CA8 rabbit polyclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 37 kDa
Observed band size: 37 kDa
Anti-CA8 rabbit polyclonal antibody at 1:500 dilution.
Lane A: A549 Membrane LysateLysates/proteins at 30 ug per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:33 kDa
Observed band size:38 kDa
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|