Anti-CA8 Antibody

Cat: 10469-RP02
Contact us:
All CA8 Reagents
CRO Services
Product recommended

All CA8 Reagents
Anti-CA8 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CA8 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human CA8
Recombinant Human Carbonic Anhydrase VIII / CA8 protein (Catalog#10469-H08E)
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VIII / CA8 (rh Carbonic Anhydrase VIII / CA8; Catalog#10469-H08E; NP_004047.3; Met 1-Gln 290). Carbonic Anhydrase VIII / CA8 specific IgG was purified by Human Carbonic Anhydrase VIII / CA8 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CA8 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CA8 Antibody (Rabbit Polyclonal antibody) Images
Human Carbonic Anhydrase VIII/CA8 Immunoprecipitation(IP) 7991

CA8 was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

2 µL anti-CA8 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CA8 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 37 kDa

Observed band size: 37 kDa

Human Carbonic Anhydrase VIII/CA8 Western blot (WB) 13232

Anti-CA8 rabbit polyclonal antibody at 1:500 dilution.

Lane A: A549 Membrane Lysate

Lysates/proteins at 30 ug per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:38 kDa

Anti-CA8 Antibody Alternative Names
Anti-CA-VIII Antibody;Anti-CALS Antibody;Anti-CAMRQ3 Antibody;Anti-CARP Antibody;Anti-MGC120502 Antibody;Anti-MGC99509 Antibody
CA8 Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

Full Name
carbonic anhydrase VIII
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CA8 Antibody Rabbit Polyclonal ELISA 50767-RP01
    Anti-CA8 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50767-T60
    Anti-CA8 Antibody Rabbit Monoclonal ELISA,ELISA(Det) 50767-R001
    Anti-CA8 Antibody Rabbit Monoclonal WB,IP 50767-R005
    Anti-CA8 Antibody Rabbit Monoclonal ELISA(Cap) 50767-R057
    Anti-CA8 Antibody Rabbit Polyclonal ELISA 10469-RP01
    Anti-CA8 Antibody Mouse Monoclonal ELISA 10469-MM09
    Anti-CA8 Antibody Rabbit Monoclonal WB,IP 10469-R008
    info info
    添加购物车成功! 添加购物车失败!