Anti-CA8 Antibody

Cat: 10469-RP02
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Anti-CA8 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CA8 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human CA8
Immunogen
Recombinant Human Carbonic Anhydrase VIII / CA8 protein (Catalog#10469-H08E)
Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VIII / CA8 (rh Carbonic Anhydrase VIII / CA8; Catalog#10469-H08E; NP_004047.3; Met 1-Gln 290). Carbonic Anhydrase VIII / CA8 specific IgG was purified by Human Carbonic Anhydrase VIII / CA8 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CA8 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CA8 Antibody (Rabbit Polyclonal antibody) Images
Human Carbonic Anhydrase VIII/CA8 Immunoprecipitation(IP) 7991

CA8 was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

2 µL anti-CA8 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CA8 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 37 kDa

Observed band size: 37 kDa

Human Carbonic Anhydrase VIII/CA8 Western blot (WB) 13232

Anti-CA8 rabbit polyclonal antibody at 1:500 dilution.

Lane A: A549 Membrane Lysate

Lysates/proteins at 30 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:38 kDa

Anti-CA8 Antibody Alternative Names
Anti-CA-VIII Antibody;Anti-CALS Antibody;Anti-CAMRQ3 Antibody;Anti-CARP Antibody;Anti-MGC120502 Antibody;Anti-MGC99509 Antibody
CA8 Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

Full Name
carbonic anhydrase VIII
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CA8 Antibody Rabbit Polyclonal ELISA 50767-RP01
    Anti-CA8 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50767-T60
    Anti-CA8 Antibody Rabbit Monoclonal ELISA,ELISA(Det) 50767-R001
    Anti-CA8 Antibody Rabbit Monoclonal WB,IP 50767-R005
    Anti-CA8 Antibody Rabbit Monoclonal ELISA(Cap) 50767-R057
    Anti-CA8 Antibody Rabbit Polyclonal ELISA 10469-RP01
    Anti-CA8 Antibody Mouse Monoclonal ELISA 10469-MM09
    Anti-CA8 Antibody Rabbit Monoclonal WB,IP 10469-R008
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