Carbonic Anhydrase VIII / CA8 Antibody

Carbonic Anhydrase VIII / CA8 Antibody Datasheet

Carbonic Anhydrase VIII / CA8 Antibody Product Information

• Immunogen: Recombinant Human Carbonic Anhydrase VIII / CA8 protein (Catalog#10469-H08E)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 1G5G12
• Ig Type: Mouse IgG2a
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase VIII / CA8 (rh Carbonic Anhydrase VIII / CA8; Catalog#10469-H08E; NP_004047.3; Met 1-Gln 290). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Carbonic Anhydrase VIII / CA8 Antibody Usage Guide

• Specificity: Human Carbonic Anhydrase VIII / CA8
• No cross-reactivity in ELISA with

  E.coli cell lysate

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CA8 in WB. Using a DAB detection system, the detection limit for Human CA8 is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CA8. The detection limit for Human CA8 is approximately 0.16 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Carbonic Anhydrase VIII / CA8 Antibody Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site. Carbonic anhydrase-related protein, also known as CARP, Carbonic anhydrase VIII, CA8 and CALS, is a member of thealpha-carbonic anhydrase family. CA8 was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, CA8 lacks carbonic anhydrase activity. CA8 continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase family.

References

1. Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95: 12596-601.
2. Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99: 16899-903.
3. Liao, S.Y. et al., 2003, J. Med. Genet. 40: 257-62.
4. Bataller,L. et al., 2004, Ann Neuro. l56 (4): 575-9.