Carbonic Anhydrase VA / CA5A Antibody (Antigen Affinity Purified)

Carbonic Anhydrase VA / CA5A Antibody ( Antigen Affinity Purified ) Datasheet

Carbonic Anhydrase VA / CA5A Antibody Product Information

• Immunogen: Recombinant Human Carbonic Anhydrase VA / CA5A protein (Catalog#10500-H08E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VA / CA5A (rh Carbonic Anhydrase VA / CA5A; Catalog#10500-H08E; NP_001730.1; Ala 40-Ser 305). Carbonic Anhydrase VA / CA5A specific IgG was purified by Human Carbonic Anhydrase VA / CA5A affinity chromatography.

Carbonic Anhydrase VA / CA5A Antibody Usage Guide

• Specificity: Human Carbonic Anhydrase VA / CA5A
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CA5A in WB. Using a DAB detection system, the detection limit for Human CA5A is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CA5A. The detection limit for Human CA5A is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Carbonic Anhydrase VA / CA5A Antibody Background

Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of thealpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).

References

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