Anti-Carbonic Anhydrase IX Antibody

Cat: 50660-RP02
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Anti-Carbonic Anhydrase IX Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Carbonic Anhydrase IX Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Mouse
Specificity
Mouse Carbonic Anhydrase IX
Immunogen
Recombinant Mouse Carbonic Anhydrase IX / CAR9 protein (Catalog#50660-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase IX / CAR9 (rM Carbonic Anhydrase IX / CAR9; Catalog#50660-M08H; NP_647466.2; Met 1-Asp 390). Carbonic Anhydrase IX / CAR9 specific IgG was purified by Mouse Carbonic Anhydrase IX / CAR9 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carbonic Anhydrase IX Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:2500-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carbonic Anhydrase IX Antibody (Rabbit Polyclonal antibody) Images
Mouse Carbonic Anhydrase IX/CA9 Immunohistochemistry(IHC) 3360
Immunochemical staining of mouse CAR9 in mouse stomach with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Mouse Carbonic Anhydrase IX/CA9 Immunohistochemistry(IHC) 3362
Immunochemical staining of mouse CAR9 in mouse intestine with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Mouse Carbonic Anhydrase IX/CA9 Western blot (WB) 6779

Anti-CA9 rabbit polyclonal antibody at 1:500 dilution.

Lane A: MOLT4 Membrane Lysate

Lysates/proteins at 20 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:50 kDa

(We are unsure as to the identity of these extra bands.)

Carbonic Anhydrase IX Background Information

Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
carbonic anhydrase IX
References
  • Loncaster JA, et al. (2001) Carbonic anhydrase (CA IX) expression, a potential new intrinsic marker of hypoxia: correlations with tumor oxygen measurements and prognosis in locally advanced carcinoma of the cervix. Cancer Res. 61(17): 6394-9.
  • Zvada J, et al. (2003) Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients. Br J Cancer. 89(6): 1067-71.
  • Pan P, et al. (2006) Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice. J Physiol. 571(Pt 2): 319-27.
  • Zhou GX, et al. (2010) Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology. 75(2): 257-61.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal WB,ELISA,IP 70028-T52
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal ELISA 70028-RP01
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal ELISA,IHC-P 50660-T24
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal IHC-P 50660-R001
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal ELISA 50660-RP01
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal ELISA,IHC-P 10107-RP02
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal ELISA,IHC-P 10107-R005
    Anti-Carbonic Anhydrase IX Antibody (FITC) Rabbit Monoclonal FCM 10107-R053-F
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal ELISA 10107-RP01
    Anti-Carbonic Anhydrase IX Antibody Mouse Monoclonal ELISA 10107-MM02
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal ELISA 10107-R011
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal WB,ELISA 10107-T48
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal WB,ELISA,IP 10107-R003
    Anti-Carbonic Anhydrase IX Antibody (PE) Rabbit Monoclonal FCM 10107-R053-P
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal ELISA,FCM 10107-R036
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal FCM 10107-R053
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