|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CA9 was immunoprecipitated using:
Lane A:0.5 mg A431 Whole Cell Lysate2 µL anti-CA9 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CA9 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 40 kDa
Observed band size: 40 kDa
Anti-CA9 rabbit monoclonal antibody at 1:500 dilution
Lane A: A431 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:50 kDa
Observed band size:40 kDa
Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Polyclonal||WB,ELISA,IP||70028-T52|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Polyclonal||ELISA||70028-RP01|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Polyclonal||ELISA,IHC-P||50660-T24|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Monoclonal||IHC-P||50660-R001|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Polyclonal||ELISA||50660-RP01|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Polyclonal||ELISA,IHC-P||10107-RP02|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Monoclonal||ELISA,IHC-P||10107-R005|
|Anti-Carbonic Anhydrase IX Antibody (FITC)||Rabbit||Monoclonal||FCM||10107-R053-F|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Polyclonal||ELISA||10107-RP01|
|Anti-Carbonic Anhydrase IX Antibody||Mouse||Monoclonal||ELISA||10107-MM02|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Monoclonal||ELISA||10107-R011|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Polyclonal||WB,ELISA||10107-T48|
|Anti-Carbonic Anhydrase IX Antibody (PE)||Rabbit||Monoclonal||FCM||10107-R053-P|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Monoclonal||ELISA,FCM||10107-R036|
|Anti-Carbonic Anhydrase IX Antibody||Rabbit||Monoclonal||FCM||10107-R053|