Anti-Carbonic Anhydrase IX Antibody

Cat: 10107-R003
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Anti-Carbonic Anhydrase IX Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Carbonic Anhydrase IX Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Carbonic Anhydrase IX
Immunogen
Recombinant Human CAIX protein (Catalog#10107-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human carbonic anhydrase IX (rhCAIX; Catalog#10107-H08H; NP_001207.2; Met 1-Asp 414).
Source
Monoclonal Rabbit IgG Clone #3
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carbonic Anhydrase IX Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carbonic Anhydrase IX Antibody (Rabbit Monoclonal antibody) Images
Human S100A7 Immunoprecipitation(IP) 8057

CA9 was immunoprecipitated using:

Lane A:0.5 mg A431 Whole Cell Lysate

2 µL anti-CA9 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CA9 rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 40 kDa

Observed band size: 40 kDa

Human Carbonic Anhydrase IX/CA9 Western blot (WB) 13120

Anti-CA9 rabbit monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:50 kDa

Observed band size:40 kDa

Anti-Carbonic Anhydrase IX Antibody Alternative Names
Anti-CAIX Antibody;Anti-Carbonic Anhydrase IX Antibody;Anti-MN Antibody
Carbonic Anhydrase IX Background Information

Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
carbonic anhydrase IX
References
  • Loncaster JA, et al. (2001) Carbonic anhydrase (CA IX) expression, a potential new intrinsic marker of hypoxia: correlations with tumor oxygen measurements and prognosis in locally advanced carcinoma of the cervix. Cancer Res. 61(17): 6394-9.
  • Zvada J, et al. (2003) Soluble form of carbonic anhydrase IX (CA IX) in the serum and urine of renal carcinoma patients. Br J Cancer. 89(6): 1067-71.
  • Pan P, et al. (2006) Carbonic anhydrase gene expression in CA II-deficient (Car2-/-) and CA IX-deficient (Car9-/-) mice. J Physiol. 571(Pt 2): 319-27.
  • Zhou GX, et al. (2010) Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials. Urology. 75(2): 257-61.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal WB,ELISA,IP 70028-T52
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal ELISA 70028-RP01
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal ELISA,IHC-P 50660-T24
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal IHC-P 50660-R001
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal ELISA 50660-RP01
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal ELISA,IHC-P 10107-RP02
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal ELISA,IHC-P 10107-R005
    Anti-Carbonic Anhydrase IX Antibody (FITC) Rabbit Monoclonal FCM 10107-R053-F
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal ELISA 10107-RP01
    Anti-Carbonic Anhydrase IX Antibody Mouse Monoclonal ELISA 10107-MM02
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal ELISA 10107-R011
    Anti-Carbonic Anhydrase IX Antibody Rabbit Polyclonal WB,ELISA 10107-T48
    Anti-Carbonic Anhydrase IX Antibody (PE) Rabbit Monoclonal FCM 10107-R053-P
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal ELISA,FCM 10107-R036
    Anti-Carbonic Anhydrase IX Antibody Rabbit Monoclonal FCM 10107-R053
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