Anti-Carbonic Anhydrase IV Antibody

Cat: 50350-RP02
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Anti-Carbonic Anhydrase IV Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Carbonic Anhydrase IV Antibody
Validated applications
IHC-P
Species reactivity
Reacts with: Mouse
Specificity
Mouse Carbonic Anhydrase IV
Immunogen
Recombinant Mouse Carbonic Anhydrase IV / Car4 / CA4 protein (Catalog#50350-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase IV / Car4 / CA4 (rM Carbonic Anhydrase IV / Car4 / CA4; Catalog#50350-M08H; NP_031633.1; Met 1-Ser 277). Carbonic Anhydrase IV / Car4 / CA4 specific IgG was purified by Mouse Carbonic Anhydrase IV / Car4 / CA4 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carbonic Anhydrase IV Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carbonic Anhydrase IV Antibody (Rabbit Polyclonal antibody) Images
Mouse Carbonic Anhydrase IV / Car4 / CA4 Immunohistochemistry(IHC) 3363
Immunochemical staining of mouse CAR4 in mouse-kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Anti-Carbonic Anhydrase IV Antibody Alternative Names
Anti-AW456718 Antibody;Anti-Ca4 Antibody;Anti-Car4 Antibody;Anti-RP23-167D6.1 Antibody
Carbonic Anhydrase IV Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

Full Name
carbonic anhydrase IV
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Baird TT, et al. (1997) Catalysis and Inhibition of Human Carbonic Anhydrase IV. Biochemistry. 36 (9): 2669-78.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Carbonic Anhydrase IV Antibody Rabbit Polyclonal ELISA 50350-RP01
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    Anti-Carbonic Anhydrase IV Antibody Rabbit Polyclonal WB,IHC-P,IP 10472-RP02
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