Anti-Carbonic Anhydrase III Antibody

Cat: 10503-RP02
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Anti-Carbonic Anhydrase III Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Carbonic Anhydrase III Antibody
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human Carbonic Anhydrase III
Immunogen
Recombinant Human Carbonic Anhydrase III / CA3 protein (Catalog#10503-H08E)
Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase III / CA3 (rh Carbonic Anhydrase III / CA3; Catalog#10503-H08E; Met 1-Lys 260; NP_005172.1). Carbonic Anhydrase III / CA3 specific IgG was purified by Human Carbonic Anhydrase III / CA3 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carbonic Anhydrase III Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carbonic Anhydrase III Antibody (Rabbit Polyclonal antibody) Images
Human Carbonic Anhydrase III Immunohistochemistry(IHC) 4627
Immunochemical staining of human CA3 in human tonsil with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to smooth muscle of blood vessel.
Human Carbonic Anhydrase III Immunohistochemistry(IHC) 4628
Immunochemical staining of human CA3 in human stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to smooth muscle.
Human Carbonic Anhydrase III Immunohistochemistry(IHC) 4629
Immunochemical staining of human CA3 in human spleen with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to smooth muscle of blood vessel.
Anti-Carbonic Anhydrase III Antibody Alternative Names
Anti-CAIII Antibody;Anti-Car3 Antibody
Carbonic Anhydrase III Background Information

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site.

Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.

Full Name
carbonic anhydrase III
References
  • Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596-601.
  • Sowden J. et al., 1998, Gene 214:157- 65.
  • Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899-903.
  • Liao, S.Y. et al., 2003, J. Med. Genet. 40: 257-62.
  • Bataller,L. et al., 2004, Ann Neurol  56 (4): 575-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Carbonic Anhydrase III Antibody Rabbit Monoclonal IHC-P 10503-R002
    Anti-Carbonic Anhydrase III Antibody Rabbit Polyclonal ELISA 10503-RP01
    Anti-Carbonic Anhydrase III Antibody Mouse Monoclonal ELISA 10503-MM09
    Anti-Carbonic Anhydrase III Antibody Rabbit Monoclonal WB,IP 10503-R006
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