Anti-Carbonic Anhydrase III Antibody

Cat: 10503-MM09
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Anti-Carbonic Anhydrase III Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-Carbonic Anhydrase III Antibody
Validated applications
Species reactivity
Reacts with: Human
Human Carbonic Anhydrase III
No cross-reactivity in ELISA with Human Carbonic Anhydrase II / CA2 Human Carbonic Anhydrase VA / CA5a Human Carbonic Anhydrase VIII / CA8 Human Carbonic Anhydrase IX / CA9 Human Carbonic Anhydrase XIII / CA13 Human Carbonic Anhydrase XII / CA12 E.coli cell lysate
Recombinant Human Carbonic Anhydrase III / CA3 Protein (Catalog#10503-H08E)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carbonic Anhydrase III / CA3 (rh Carbonic Anhydrase III / CA3; Catalog#10503-H08E; Met 1-Lys 260; NP_005172.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #5G8B6
Protein A
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carbonic Anhydrase III Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carbonic Anhydrase III Antibody Alternative Names
Anti-CAIII Antibody;Anti-Car3 Antibody
Carbonic Anhydrase III Background Information

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site.

Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.

Full Name
carbonic anhydrase III
  • Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596-601.
  • Sowden J. et al., 1998, Gene 214:157- 65.
  • Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899-903.
  • Liao, S.Y. et al., 2003, J. Med. Genet. 40: 257-62.
  • Bataller,L. et al., 2004, Ann Neurol  56 (4): 575-9.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Carbonic Anhydrase III Antibody Rabbit Polyclonal ELISA,IHC-P 10503-RP02
    Anti-Carbonic Anhydrase III Antibody Rabbit Monoclonal IHC-P 10503-R002
    Anti-Carbonic Anhydrase III Antibody Rabbit Polyclonal ELISA 10503-RP01
    Anti-Carbonic Anhydrase III Antibody Rabbit Monoclonal WB,IP 10503-R006
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