Anti-Carbonic anhydrase II Antibody

Cat: 50685-RP02
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All Carbonic anhydrase II Reagents
CRO Services
Product recommended

All Carbonic anhydrase II Reagents
Anti-Carbonic anhydrase II Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Carbonic anhydrase II Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse Carbonic anhydrase II
Immunogen
Recombinant Mouse Carbonic Anhydrase II / CA2 protein (Catalog#50685-M08E)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase II / CA2 (rM Carbonic Anhydrase II / CA2; Catalog#50685-M08E; AAH55291.1; Met 1-Lys 260). Carbonic Anhydrase II / CA2 specific IgG was purified by Mouse Carbonic Anhydrase II / CA2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carbonic anhydrase II Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IHC-P 1:2500-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carbonic anhydrase II Antibody (Rabbit Polyclonal antibody) Images
Mouse Carbonic Anhydrase II Immunohistochemistry(IHC) 3368
Immunochemical staining of mouse CA2 in mouse stomach with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Mouse Carbonic Anhydrase II Immunohistochemistry(IHC) 3370
Immunochemical staining of mouse CA2 in mouse kidney with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Mouse Carbonic Anhydrase II Immunohistochemistry(IHC) 3372
Immunochemical staining of mouse CA2 in mouse intestine with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
Mouse Carbonic Anhydrase II Immunohistochemistry(IHC) 3374
Immunochemical staining of human CK14 in rat skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Mouse Carbonic Anhydrase II Immunoprecipitation(IP) 8248

Mouse CA2 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

2 µL anti-Mouse CA2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse CA2 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 29 kDa

Observed band size: 29 kDa

Mouse Carbonic Anhydrase II Western blot (WB) 13698

Anti-CA2 rabbit polyclonal antibody at 1:500 dilution

Lane A: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:29 kDa

Anti-Carbonic anhydrase II Antibody Alternative Names
Anti-AI131712 Antibody;Anti-Ca2 Antibody;Anti-CAII Antibody;Anti-Car-2 Antibody;Anti-Ltw-5 Antibody;Anti-Lvtw-5 Antibody
Carbonic anhydrase II Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

Full Name
carbonic anhydrase II
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.
  • Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Carbonic anhydrase II Antibody Rabbit Monoclonal IHC-P 50685-R003
    Anti-Carbonic anhydrase II Antibody Rabbit Monoclonal ELISA 50685-R002
    Anti-Carbonic anhydrase II Antibody Rabbit Polyclonal ELISA 50685-RP01
    Anti-Carbonic anhydrase II Antibody Rabbit Polyclonal ELISA 10478-RP01
    Anti-Carbonic anhydrase II Antibody Rabbit Monoclonal ELISA 10478-R004
    Anti-Carbonic anhydrase II Antibody Rabbit Monoclonal WB,IP 10478-R001
    Anti-Carbonic anhydrase II Antibody Rabbit Polyclonal WB,ELISA,IP 10478-RP02
    info info
    添加购物车成功! 添加购物车失败!