Carbonic Anhydrase II / CA2 Antibody (Antigen Affinity Purified)

Carbonic Anhydrase II / CA2 Antibody ( Antigen Affinity Purified ) Datasheet

Carbonic Anhydrase II / CA2 Antibody Product Information

• Immunogen: Recombinant Mouse Carbonic Anhydrase II / CA2 protein (Catalog#50685-M08E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase II / CA2 (rM Carbonic Anhydrase II / CA2; Catalog#50685-M08E; AAH55291.1; Met 1-Lys 260). Carbonic Anhydrase II / CA2 specific IgG was purified by Mouse Carbonic Anhydrase II / CA2 affinity chromatography.

Carbonic Anhydrase II / CA2 Antibody Usage Guide

• Specificity: Mouse Carbonic Anhydrase II / CA2
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of mouse CA2 in mouse kidney with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of mouse CA2 in mouse brain with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).

  

  Immunochemical staining of mouse CA2 in mouse stomach with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).

  Immunochemical staining of mouse CA2 in mouse intestine with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CA2 in WB. Using a DAB detection system, the detection limit for Mouse CA2 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CA2. The detection limit for Mouse CA2 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Carbonic Anhydrase II / CA2 Antibody Background

Carbonic anhydrases (Cas) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site. Mouse carbonic anhydrase 2, also known as Carbonic anhydrase II, Carbonate dehydratase II, Carbonic anhydrase C and CA2, is cytoplasm protein which belongs to thealpha-carbonic anhydrase family. CA2 is essential for bone resorption and osteoclast differentiation. CA2 can hydrates cyanamide to urea. It is involved in the regulation of fluid secretion into the anterior chamber of the eye. Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3) also known as osteopetrosis with renal tubular acidosis, carbonic anhydrase II deficiency syndrome, Guibaud-Vainsel syndrome or marble brain disease. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone.

References

1. Briganti F. et al.,1999, J. Biol. Inorg. Chem. 4: 528-36.
2. Cox J.D. et al., 2000, Biochemistry 39: 13687-94.
3. Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99: 16899-903.
4. Liao, S.Y. et al., 2003, J. Med. Genet. 40: 257-62.
5. Loiselle F.B. et al., 2004, Am. J. Physiol. 286: C1423-C33.
6. Pushkin A. et al., 2004, J. Physiol. (Lond.) 559: 55-65.