|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CA2 was immunoprecipitated using:
Lane A:0.5 mg 293T Whole Cell Lysate
Lane B:0.5 mg Caco-2 Whole Cell Lysate2 µL anti-CA2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CA2 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 29 kDa
Observed band size: 29 kDa
Anti-CA2 rabbit polyclonal antibody at 1:500 dilution
Lane A: HEK293 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:29 kDa
Observed band size:30 kDa
(We are unsure as to the identity of these extra bands.)
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Carbonic anhydrase II Antibody||Rabbit||Monoclonal||IHC-P||50685-R003|
|Anti-Carbonic anhydrase II Antibody||Rabbit||Polyclonal||WB,ELISA,IHC-P,IP||50685-RP02|
|Anti-Carbonic anhydrase II Antibody||Rabbit||Monoclonal||ELISA||50685-R002|
|Anti-Carbonic anhydrase II Antibody||Rabbit||Polyclonal||ELISA||50685-RP01|
|Anti-Carbonic anhydrase II Antibody||Rabbit||Polyclonal||ELISA||10478-RP01|
|Anti-Carbonic anhydrase II Antibody||Rabbit||Monoclonal||ELISA||10478-R004|
|Anti-Carbonic anhydrase II Antibody||Rabbit||Monoclonal||WB,IP||10478-R001|