Anti-Carbonic anhydrase II Antibody

Cat: 10478-RP02
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Anti-Carbonic anhydrase II Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-Carbonic anhydrase II Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Carbonic anhydrase II
Immunogen
Recombinant Human Carbonic Anhydrase II / CA2 protein (Catalog#10478-H08E)
Preparation
Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase II / CA2 (rh Carbonic Anhydrase II / CA2; Catalog#10478-H08E; NP_000058.1; Met 1-Lys 260). Carbonic Anhydrase II / CA2 specific IgG was purified by Human Carbonic Anhydrase II / CA2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Carbonic anhydrase II Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Carbonic anhydrase II Antibody (Rabbit Polyclonal antibody) Images
Human Carbonic Anhydrase II Immunoprecipitation(IP) 7994

CA2 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg Caco-2 Whole Cell Lysate

2 µL anti-CA2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CA2 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 29 kDa

Observed band size: 29 kDa

Human Carbonic Anhydrase II Western blot (WB) 13235

Anti-CA2 rabbit polyclonal antibody at 1:500 dilution

Lane A: HEK293 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:30 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Carbonic anhydrase II Antibody Alternative Names
Anti-CA-II Antibody;Anti-CAC Antibody;Anti-CAII Antibody;Anti-Car2 Antibody;Anti-HEL-76 Antibody;Anti-HEL-S-282 Antibody
Carbonic anhydrase II Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

Full Name
carbonic anhydrase II
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.
  • Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
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