Canine RBP4 Protein (His Tag)

Retinol binding protein 4, plasma Protein Datasheet

RBP4 Protein Product Information

• Synonym: RBP4
• Protein Construction: A DNA sequence encoding the canine RBP4 (F1PQI4) (Met1-Leu201) was expressed with a C-terminal polyhistidine tag.
• Source: Rat
• Expression Host: Human Cells

RBP4 Protein QC Testing

• Purity: >94% as determined by SDS-PAGE
• Bio-activity: Measured by its ability to bind alltrans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4.
  The 50% binding concentration (BC50) is > 5 μM.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 19
• Molecular Mass: The recombinant canine RBP4 comprises 194 amino acids and has a predicted molecular mass of 22.4 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: RBP4 protein

RBP4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

RBP4 Protein Description

Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. In addition, RBP4 is recently identified as an adipokine whose dysregulation is closely associated with the pathogenesis of insulin resistance and type 2 diabetes, as well as obesity.

References

1. Colantuoni V. et al., 1983, Nucleic acid Res. 11: 7769-76.
2. Loredana Q. et al., 2002, J Biol Chem. 277:30191-7.
3. Zanotti G. et al., 2004, Vitamins and Hormones. 69: 271-95.
4. Cho YM. et al., 2006, Diabetes Care. 29: 2457-61.
5. Balagopal P. et al., 2007, J Clin Endocirnol Metab. 92: 1971-4.