|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine BMP-5 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||DG70075-ACG|
|Canine BMP-5 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||DG70075-ACR|
|Canine BMP-5 Gene ORF cDNA clone expression plasmid, C-Flag tag||DG70075-CF|
|Canine BMP-5 Gene ORF cDNA clone expression plasmid, C-His tag||DG70075-CH|
|Canine BMP-5 Gene ORF cDNA clone expression plasmid, C-Myc tag||DG70075-CM|
|Canine BMP-5 Gene ORF cDNA clone expression plasmid, C-HA tag||DG70075-CY|
|Canine BMP-5 Gene ORF cDNA clone in cloning vector||DG70075-G|
|Canine BMP-5 Gene ORF cDNA clone expression plasmid, N-Flag tag||DG70075-NF|
|Canine BMP-5 Gene ORF cDNA clone expression plasmid, N-His tag||DG70075-NH|
|Canine BMP-5 Gene ORF cDNA clone expression plasmid, N-Myc tag||DG70075-NM|
|Canine BMP-5 Gene ORF cDNA clone expression plasmid, N-HA tag||DG70075-NY|
|Canine BMP-5 Gene ORF cDNA clone expression plasmid||DG70075-UT|
|Learn more about expression Vectors|
Bone Morphogenetic Protein 5 (BMP-5) is a member of the structurally and functionally related bone morphogenetic proteins (BMPs) which constitute a novel subfamily of the transforming growth factor β (TGF-β) superfamily. In agreement with a possible role in the control of cell death, BMP-5 exhibited a regulated pattern of expression in the interdigital tissue. Transcripts of BMP-5 and BMP-5 protein were abundant within the cytoplasm of the fragmenting apoptotic interdigital cells in a way suggesting that delivery of BMPs into the tissue is potentiated during apoptosis. Gain-of-function experiments demonstrated that BMP-5 has the same effect as other interdigital BMPs inducing apoptosis in the undifferentiated mesoderm and growth in the prechondrogenic mesenchyme. BMP-5 is a member of the 60A subgroup of BMPs, other members of which have been shown to stimulate dendritic growth in central and peripheral neurons. The signaling pathway that mediates the dendrite-promoting activity of BMP-5 may involve binding to BMPR-IA and activation of Smad-1, and relative levels of BMP antagonists such as noggin and follistatin may modulate BMP-5 signaling. Since BMP-5 is expressed at relatively high levels not only in the developing but also the adult nervous system, these findings suggest the possibility that BMP-5 regulates dendritic morphology not only in the developing, but also the adult nervous system. BMP-5 may play important roles not only in myocardial differentiation, but also in the formation and maintenance of endocardial cushion tissue. Additionally, high expression level of BMP-5 has been detected in certain tumors of mesenchymal origin.