Mouse CDH6/KCAD Gene cDNA Clone / ORF Clone

CDH6 cDNA Clone Datasheet

CDH6 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007666.3
• cDNA Size: 2373
• Gene Synonym: cad6; MGC129338; MGC129360; K-cadherin; cadherin-6; Cdh6
• Source: Mouse

CDH6 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cadherin 6 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for six point mutations: 1600 A/G resulting in the amino acid Ser substitution by Gly, 96 A/G, 1236 T/C, 1311 T/C, 1899 C/T and 2319 G/A not causing the amino acid variation.

CDH6 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDH6 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CDH6 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAACTT ACCGGTACTT CTTGCTGCTC TTTTGGGTCG GCCAGCCCTA CCCAACTTTC
61       TCAAACCCAT TATCTAAAAG GACTAGTGGC TTCCCGGCAA AGAGGAAAGC CCTGGAGCTC
121      TCTGCAAACA GCAGGAATGA GCTGAGCCGT TCGAAAAGGA GTTGGATGTG GAATCAGTTC
181      TTCCTCCTGG AGGAATACAC GGGATCCGAT TATCAGTACG TGGGCAAGTT ACATTCAGAC
241      CAGGATAGAG GAGATGGATC ACTTAAATAT ATCCTTTCAG GAGATGGAGC GGGCGATCTC
301      TTCATTATCA ATGAGAACAC AGGCGACATA CAGGCCACCA AGAGGCTGGA CAGGGAAGAA
361      AAACCTGTTT ACATCCTTCG AGCTCAAGCT GTCAACAGAA GGACAGGGAG ACCCGTCGAG
421      CCTGAGTCTG AATTCATCAT CAAGATCCAC GACATCAATG ACAATGAGCC AATATTCACC
481      AAGGACGTTT ACACAGCCAC AGTCCCGGAG ATGGCTGATG TTGGCACATT TGTGGTCCAA
541      GTCACGGCGA CTGATGCTGA CGACCCAACA TATGGGAACA GTGCTAAAGT CGTCTATAGC
601      ATCCTGCAGG GACAGCCCTA CTTTTCAGTG GAATCGGAAA CAGGTATCAT CAAGACAGCG
661      TTGCTCAACA TGGATCGAGA AAACAGAGAA CAGTACCAAG TGGTGATTCA GGCCAAGGAC
721      ATGGGCGGCC AGATGGGAGG ACTGTCGGGG ACTACGACCG TGAACATCAC GCTGACAGAT
781      GTCAATGACA ACCCACCCCG TTTCCCCCAG AGTACCTACC AGTTTAAGAC CCCCGAGTCC
841      TCTCCACCGG GAACGCCAAT TGGCAGGATC AAAGCCAGTG ATGCCGATGT GGGAGAAAAT
901      GCGGAGATCG AGTACAGCAT CACTGACGGT GAGGGACACG AGATGTTTGA TGTCATCACC
961      GACCAGGAAA CCCAGGAAGG GATCATAACT GTCAAAAAGC TCTTGGATTT TGAGAAGAAG
1021     AAGGTGTACA CCCTCAAGGT GGAAGCCTCC AATCCCCACG TCGAACCCCG ATTTCTCTAC
1081     CTGGGTCCCT TCAAAGACTC GGCCACGGTG AGAATCGTGG TGGACGATGT GGACGAACCT
1141     CCTGTCTTCA GCAAACTGGC CTACATCCTA CAGATACGGG AGGATGCCCG GATAAATACG
1201     ACCATAGGCT CTGTCGCAGC TCAGGATCCT GACGCCGCCA GGAATCCTGT CAAGTATTCT
1261     GTGGATAGAC ACACAGATAT GGACAGGATA TTCAACATTG ATTCTGGAAA CGGTTCGATT
1321     TTTACATCAA AACTTCTCGA CCGGGAAACC CTGCTGTGGC ACAACATCAC AGTGATCGCA
1381     ACGGAGATTA ATAACCCAAA GCAAAGCAGC CGCGTTCCTC TATATATTAA AGTTCTCGAC
1441     GTCAACGACA ATGCCCCAGA GTTTGCTGAG TTCTATGAAA CTTTTGTCTG TGAAAAAGCA
1501     AAGGCAGATC AGCTGATTCA GACCCTGCGT GCTGTTGACA AGGATGATCC TTACAGCGGA
1561     CACCAATTTT CCTTCTCCTT GGCCCCAGAA GCAGCCAGCG GCTCAAACTT TACTATACAA
1621     GACAACAAAG ACAACACTGC AGGGATCCTA ACTCGGAAAA ATGGCTATAA CAGACACGAG
1681     ATGAGCACCT ATCTCCTGCC TGTGGTCATT TCGGACAACG ACTACCCAGT CCAAAGCAGC
1741     ACTGGGACAG TGACTGTCCG GGTCTGTGCA TGTGACCACC ATGGGAACAT GCAGTCCTGC
1801     CACGCCGAGG CCCTCATCCA CCCCACAGGG CTGAGCACAG GGGCTCTGGT GGCCATCCTT
1861     CTGTGCATCG TGATCCTACT AGTGACAGTG GTGCTGTTTG CAGCTCTGAG GCGGCAGCGA
1921     AAGAAAGAGC CTTTGATCAT TTCCAAAGAG GACATCAGAG ATAACATTGT CAGTTACAAC
1981     GACGAAGGTG GCGGAGAGGA AGACACCCAG GCCTTTGATA TCGGAACCCT GAGGAACCCT
2041     GAAGCCATGG AGGACAGCAA ATCGCGCAGG GACATTGTGC CTGAAGCTCT TTTTCTACCC
2101     CGGCGGACTC CAACAGCTCG TGACAACACG GATGTCAGAG ATTTCATTAA CCAAAGGTTA
2161     AAGGAAAATG ACACGGACCC AACAGCCCCT CCTTACGACT CCTTGGCCAC CTATGCCTAC
2221     GAAGGCACCG GCTCTGTGGC CGACTCCCTG AGCTCGCTGG AATCCGTGAC CACGGATGGA
2281     GATCAAGATT ATGACTATTT GAGTGACTGG GGCCCTCGAT TCAAAAAGCT GGCAGATATG
2341     TATGGAGGGA TGGACAGTGA CAAAGACTCC TAA

 

Quality Control:cDNA sequencing results is available upon request.