Cadherin-17 / LI-cadherin Antibody

CDH17 / Cadherin-17 Antibody Datasheet

CDH17 / Cadherin-17 Antibody Product Information

• Immunogen: Recombinant Human CDH17 / Cadherin-17 Protein (Catalog #11360-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 4F6H5
• Ig Type: Mouse IgG2a
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human CDH17 / Cadherin-17 (rh CDH17 / Cadherin-17; Catalog #11360-H08H; Met 1-Met 787; Q12864-1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CDH17 / Cadherin-17 Antibody Usage Guide

• Specificity: Human CDH17 / Cadherin-17
• No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
  

  Human Cadherin-5

  Human Cadherin-8

  Human E-Cadherin

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CDH17 in WB. Using a DAB detection system, the detection limit for Human CDH17 is approximately 2 ng/lane under non-reducing conditions and 20 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CDH17. The detection limit for Human CDH17 is approximately 0.156 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CDH17 / Cadherin-17 Antibody Background

Cadherins ( Calcium dependent adhesion molecules ) are a class of transmembrane proteins. They play important roles in cell adhesion, ensuring that cells within tissues are bound together. They are dependent on calcium ( Ca2+ ) ions to function. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I  cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development.
Cadherin-17, also known as Intestinal peptide-associated transporter HPT-1, Liver-intestine cadherin, LI-cadherin and CDH17, is a single-pass type I  membrane protein which contains 7 cadherin domains. Cadherin-17 / CDH17 is expressed in the gastrointestinal tract and pancreatic duct. It is not detected in kidney, lung, liver, brain, adrenal gland and skin. The expression of Cadherin-17 / CDH17 is characteristic of the advanced gastric carcinoma that is associated with poor prognosis. Cadherin-17 / CDH17 is a structurally unique member of the cadherin superfamily.

References

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3. Duguay, D. et al., 2003, Dev. Biol. 253: 309-23.
4. Ito,R. et al., 2005, Virchows Arch. 447 (4):717-22.
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6. Crosby, CV, et al., 2005, Blood. 105 (7): 2771-6. 
7. Hulpiau, P. et al., 2009, Int. J. Biochem. Cell Biol. 41 (2): 349-69.