Anti-CDH17 Antibody

Cat: 11360-RP02
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Anti-CDH17 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CDH17 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human CDH17
Immunogen
Recombinant Human CDH17 / Cadherin-17 protein (Catalog#11360-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CDH17 / Cadherin-17 (rh CDH17 / Cadherin-17; Catalog#11360-H08H; Met 1-Met 787; Q12864-1). CDH17 / Cadherin-17 specific IgG was purified by Human CDH17 / Cadherin-17 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CDH17 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:2000-1:10000  
IP 1-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CDH17 Antibody (Rabbit Polyclonal antibody) Images
Human Cadherin-17/LI-cadherin Immunohistochemistry(IHC) 4447
Immunochemical staining of human CDH17 in human rectal carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of intestinal gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Human Cadherin-17/LI-cadherin Immunohistochemistry(IHC) 4451
Immunochemical staining of human CDH17 in human colon carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of colonic gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Human Cadherin-17/LI-cadherin Immunohistochemistry(IHC) 4452
Immunochemical staining of human CDH17 in human appendix with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of intestinal gland.
Human Cadherin-17/LI-cadherin Immunoprecipitation(IP) 8086

CDH17 was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

0.5 µL anti-CDH17 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CDH17 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 92 kDa

Observed band size: 92 kDa

Human Cadherin-17/LI-cadherin Western blot (WB) 13396

Anti-CDH17 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:92 kDa

Observed band size:80 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CDH17 Antibody Alternative Names
Anti-CDH16 Antibody;Anti-HPT-1 Antibody;Anti-HPT1 Antibody
CDH17 Background Information

Cadherin-17 or LI-cadherin is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Cadherin-17/LI-cadherin is a cadherin-like protein consisting of an extracellular region, 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing of the encoding gene results in multiple transcript variants. Cadherin-17/LI-cadherin preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin-17 may thus contribute to the sorting of heterogeneous cell types and have a role in the morphological organization of liver and intestine. It's also involved in intestinal peptide transport. Experiments have reported the association between Cadherin-17/LI-cadherin and gastric cancer. Cadherin-17/LI-cadherin expression was detected in 63/94 of gastric adenocarcinomas in addition to intestinal metaplasia. The expression of Cadherin-17 tended to be associated with intestinal type carcinoma, and carcinomas with Cadherin-17 expression was significantly more frequent in advanced stage cases than in early stage. Cadherin-17 is also a useful immunohistochemical marker for diagnosis of adenocarcinomas of the digestive system.

Full Name
cadherin 17, LI cadherin (liver-intestine)
References
  • Liu LX, et al. (2009) Targeting cadherin-17 inactivates Wnt signaling and inhibits tumor growth in liver carcinoma. Hepatology. 50(5): 1453-63.
  • Ito R, et al. (2005) Clinicopathological significant and prognostic influence of cadherin-17 expression in gastric cancer. Virchows Arch. 447(4): 717-22.
  • Horsfield J, et al. (2002) Cadherin-17 is required to maintain pronephric duct integrity during zebrafish development. Mech Dev. 115(1-2): 15-26.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CDH17 Antibody Rabbit Polyclonal ELISA 80283-T16
    Anti-CDH17 Antibody Rabbit Polyclonal IHC-P 107971-T08
    Anti-CDH17 Antibody Mouse Monoclonal IHC-P 11360-MM05
    Anti-CDH17 Antibody Rabbit Polyclonal ELISA 11360-RP01
    Anti-CDH17 Antibody Rabbit Monoclonal IHC-P 11360-R029
    Anti-CDH17 Antibody Rabbit Monoclonal ELISA 11360-R032
    Anti-CDH17 Antibody Mouse Monoclonal ELISA 11360-MM02
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