Cadherin-17 / LI-cadherin Antibody (Antigen Affinity Purified)

CDH17 / Cadherin-17 Antibody (Antigen Affinity Purified) Datasheet

CDH17 / Cadherin-17 Antibody Product Information

• Immunogen: Recombinant Human CDH17 / Cadherin-17 protein (Catalog #11360-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Human CDH17 / Cadherin-17 (rh CDH17 / Cadherin-17; Catalog #11360-H08H; Met 1 - Met 787; Q12864-1). CDH17 / Cadherin-17 specific IgG was purified by Human CDH17 / Cadherin-17 affinity chromatography.

CDH17 / Cadherin-17 Antibody Usage Guide

• Specificity: Human CDH17 / Cadherin-17
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of human CDH17 in human colon carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of colonic gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunochemical staining of human CDH17 in human rectal carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of intestinal gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunochemical staining of human CDH17 in human appendix with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The image showing membrane staining of intestinal gland.

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CDH17. The detection limit for Human CDH17 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CDH17 / Cadherin-17 Antibody Background

Cadherins ( Calcium dependent adhesion molecules ) are a class of transmembrane proteins. They play important roles in cell adhesion, ensuring that cells within tissues are bound together. They are dependent on calcium ( Ca2+ ) ions to function. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I  cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development.
Cadherin-17, also known as Intestinal peptide-associated transporter HPT-1, Liver-intestine cadherin, LI-cadherin and CDH17, is a single-pass type I  membrane protein which contains 7 cadherin domains. Cadherin-17 / CDH17 is expressed in the gastrointestinal tract and pancreatic duct. It is not detected in kidney, lung, liver, brain, adrenal gland and skin. The expression of Cadherin-17 / CDH17 is characteristic of the advanced gastric carcinoma that is associated with poor prognosis. Cadherin-17 / CDH17 is a structurally unique member of the cadherin superfamily.

References

1. Angst, B. et al., 2001, J Cell Sci. 114 (Pt 4): 629-41.
2. Horsfield,J. et al., 2002,Mech Dev. 115 (1-2):15-26.
3. Duguay, D. et al., 2003, Dev. Biol. 253: 309-23.
4. Ito,R. et al., 2005, Virchows Arch. 447 (4):717-22.
5. Bayas, MV. et al., 2005, Biophysical. Journal. 90 (4): 1385.
6. Crosby, CV, et al., 2005, Blood. 105 (7): 2771-6. 
7. Hulpiau, P. et al., 2009, Int. J. Biochem. Cell Biol. 41 (2): 349-69.