Cadherin-1 / CDH1 / CD324 / E-cadherin Antibody

Cadherin-1 / CDH1 / CD324 / E-cadherin Antibody Datasheet

Cadherin-1 / CDH1 / CD324 / E-cadherin Antibody Product Information

• Immunogen: Recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin protein (Catalog#10204-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 7G5D4
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin (rh Cadherin-1 / CDH1 / CD324 / E-cadherin; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Cadherin-1 / CDH1 / CD324 / E-cadherin Antibody Usage Guide

• Specificity: Human Cadherin-1 / CDH1 / CD324 / E-cadherin
• Immunochemistry: ICC/IF: 15-30 μg/mL

  

  Immunofluorescence staining of human E-cadherin in MCF-7 cells with mouse monoclonal antibody (15 μg/mL). The image showing membrane staining of MCF-7 cells.

  Immunofluorescence staining of human E-cadherin in chHES-8 cells with mouse monoclonal antibody (15μg/mL).The image showing membrane staining of chHES-8 cells.

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human E-cad.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Cadherin-1 / CDH1 / CD324 / E-cadherin Antibody Background

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is the classical cadherin molecule and contains five cadherin domains of ~110 aa each in the extracellular domain. E-Cad is expressed in the non-neural epithelial tissues as disulfide-linked homodimer, and plays a key role in the organization and integrity of most epithelial tissues, cell differentiation and tissue development. CDH1 interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas (gastric, breast, ovary, endometrium and thyroid).

References

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