Anti-E-Cadherin Antibody (FITC) (Mouse Monoclonal antibody) General Information
Product name
Anti-E-Cadherin Antibody (FITC)
Validated applications
FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human E-Cadherin
Immunogen
Recombinant Human CDH1 protein (Catalog#10204-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin (rh Cadherin-1 / CDH1 / CD324 / E-cadherin; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Mouse IgG2a Clone #08
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10 μl/Test, 0.1 mg/ml
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Anti-E-Cadherin Antibody (FITC) (Mouse Monoclonal antibody) Validated Applications
| Application |
Dilution |
Notes |
| ICC-IF |
5-20 μg/mL |
|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-E-Cadherin Antibody (FITC) (Mouse Monoclonal antibody) Images
Profile of anti-E-cad (CD324) reactivity on MCF-7 cells analyzed by flow cytometry.
Immunofluorescence staining of Human E-CAD in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human MCF7 monoclonal antibody (FITC-conjugated, 10 µg/ml) at 37℃ 1 hour, and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Anti-E-Cadherin Antibody (FITC) Alternative Names
Anti-Arc-1 Antibody;Anti-CD324 Antibody;Anti-CDH1 Antibody;Anti-CDHE Antibody;Anti-E-cad Antibody;Anti-E-Cadherin Antibody;Anti-ECAD Antibody;Anti-LCAM Antibody;Anti-UVO Antibody
E-Cadherin Background Information
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
Full Name
cadherin 1, type 1, E-cadherin (epithelial)
References
Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
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