Anti-E-Cadherin Antibody (FITC)

Cat: 10204-MM08-F
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Anti-E-Cadherin Antibody (FITC) (Mouse Monoclonal antibody) General Information
Product name
Anti-E-Cadherin Antibody (FITC)
Validated applications
FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human E-Cadherin
Immunogen
Recombinant Human CDH1 protein (Catalog#10204-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cadherin-1 / CDH1 / CD324 / E-cadherin (rh Cadherin-1 / CDH1 / CD324 / E-cadherin; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Source
Monoclonal Mouse IgG2a Clone #08
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
FITC
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Anti-E-Cadherin Antibody (FITC) (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 5-20 μg/mL  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-E-Cadherin Antibody (FITC) (Mouse Monoclonal antibody) Images
Human E-Cadherin/CDH1/E-cad/CD324 Flow Cytometry (FC) 2601
Profile of anti-E-cad (CD324) reactivity on MCF-7 cells analyzed by flow cytometry.
Human E-Cadherin/CDH1/E-cad/CD324 Immunofluorescence(IF) 2943
Immunofluorescence staining of Human E-CAD in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human MCF7 monoclonal antibody (FITC-conjugated, 10 µg/ml) at 37℃ 1 hour, and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Anti-E-Cadherin Antibody (FITC) Alternative Names
Anti-Arc-1 Antibody;Anti-CD324 Antibody;Anti-CDH1 Antibody;Anti-CDHE Antibody;Anti-E-cad Antibody;Anti-E-Cadherin Antibody;Anti-ECAD Antibody;Anti-LCAM Antibody;Anti-UVO Antibody
E-Cadherin Background Information

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Full Name
cadherin 1, type 1, E-cadherin (epithelial)
References
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA 80274-MM04
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 80274-T50
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    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50671-RP02
    Anti-E-Cadherin Antibody Rabbit Monoclonal ELISA 10204-R055
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA(Cap) 10204-MM04
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,IHC-P,ICC/IF,IF 10204-T58
    Anti-E-Cadherin Antibody Rabbit Polyclonal ELISA,IHC-P,ICC/IF,IF 10204-T26
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA 10204-MM07
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IHC-Fr 10204-RP02
    Anti-E-Cadherin Antibody (APC) Mouse Monoclonal FCM 10204-MM08-A
    Anti-E-Cadherin Antibody Mouse Monoclonal FCM 10204-MM08
    Anti-E-Cadherin Antibody Rabbit Polyclonal ELISA 10204-RP01
    Anti-E-Cadherin Antibody Rabbit Monoclonal IHC-P,ICC/IF,IF 10204-R032
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA 10204-MM12
    Anti-E-Cadherin Antibody (PE) Mouse Monoclonal FCM,ICC/IF,IF 10204-MM08-P
    Anti-E-Cadherin Antibody Rabbit Monoclonal ELISA 10204-R301
    Anti-E-Cadherin Antibody Rabbit Monoclonal WB,IP 10204-R056
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