Mouse CYR61 Gene cDNA Clone / ORF Clone

CYR61 cDNA Clone Datasheet

CYR61 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010516.2
• cDNA Size: 1140
• Gene Synonym: CCN1; Igfbp10; AI325051; Cyr61
• Source: Mouse

CYR61 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cysteine rich protein 61 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

CYR61 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CYR61 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CYR61 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCTCCA GCACCTTCAG GACGCTCGCT GTCGCCGTCA CCCTTCTCCA CTTGACCAGA
61       CTGGCGCTCT CCACCTGCCC CGCCGCCTGC CACTGCCCTC TGGAGGCACC CAAGTGCGCC
121      CCGGGAGTCG GGTTGGTCCG GGACGGCTGC GGCTGCTGTA AGGTCTGCGC TAAACAACTC
181      AACGAGGACT GCAGCAAAAC TCAGCCCTGC GACCACACCA AGGGGTTGGA ATGCAATTTC
241      GGCGCCAGCT CCACCGCTCT GAAAGGGATC TGCAGAGCTC AGTCAGAAGG CAGACCCTGT
301      GAATATAACT CCAGAATCTA CCAAAACGGG GAAAGCTTCC AGCCCAACTG TAAACACCAG
361      TGCACATGTA TTGATGGCGC CGTGGGCTGC ATTCCTCTGT GTCCCCAAGA ACTGTCTCTC
421      CCCAATCTGG GCTGTCCCAA CCCCCGGCTG GTGAAAGTCA GCGGGCAGTG CTGTGAAGAG
481      TGGGTTTGTG ATGAAGACAG CATTAAGGAC TCCCTGGACG ACCAGGATGA CCTCCTCGGA
541      CTCGATGCCT CGGAGGTGGA GTTAACGAGA AACAATGAGT TAATCGCAAT TGGAAAAGGC
601      AGCTCACTGA AGAGGCTTCC TGTCTTTGGC ACCGAACCGC GAGTTCTTTT CAACCCTCTG
661      CACGCCCATG GCCAGAAATG CATCGTTCAG ACCACGTCTT GGTCCCAGTG CTCCAAGAGC
721      TGCGGAACTG GCATCTCCAC ACGAGTTACC AATGACAACC CAGAGTGCCG CCTGGTGAAA
781      GAGACCCGGA TCTGTGAAGT GCGTCCTTGT GGACAACCAG TGTACAGCAG CCTAAAAAAG
841      GGCAAGAAAT GCAGCAAGAC CAAGAAATCC CCAGAACCAG TCAGATTTAC TTATGCAGGA
901      TGCTCCAGTG TCAAGAAATA CCGGCCCAAA TACTGCGGCT CCTGCGTAGA TGGCCGGTGC
961      TGCACACCTC TGCAGACCAG AACTGTGAAG ATGCGGTTCC GATGCGAAGA TGGAGAGATG
1021     TTTTCCAAGA ATGTCATGAT GATCCAGTCC TGCAAATGTA ACTACAACTG CCCGCATCCC
1081     AACGAGGCAT CGTTCCGACT GTACAGCCTA TTCAATGACA TCCACAAGTT CAGGGACTAA

 

Quality Control:cDNA sequencing results is available upon request.