+86-400-890-9989
Product Catalog
Research Topics
Your Position: Home
> Gene Products > Human cDNA Clones > Human CYR61 ORF Expression Clone, FLAG-tagged Human CYR61 ORF Expression Clone, FLAG-tagged
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG11606-M-F |
|
YES |  |
|
|
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
 |
|||
CYR61 cDNA Clone Datasheet
CYR61 cDNA Clone Product Information
|
Gene Bank Ref.ID: |
NM_001554.4 |
|
cDNA Size: |
1174 |
| Gene Synonym: |
CCN1;GIG1;IGFBP10 |
| Source: | Human |
CYR61 cDNA Clone Description
|
cDNA Description: |
ORF Clone of Homo sapiens cysteine-rich, angiogenic inducer, 61 DNA. |
| Vector: | |
| FLAG Tag Sequence: | GATTACAAGGATGACGACGATAAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutation 816T/C not causing the amino acid variation. |
CYR61 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
CYR61 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
CYR61 cDNA Clone Sequence and Quality Control
Sequence:
which has no influence on protein expression.
2. FLAG Tag
1 ATGAGCTCCC GCATCGCCAG GGCGCTCGCC TTAGTCGTCA CCCTTCTCCA CTTGACCAGG 61 CTGGCGCTCT CCACCTGCCC CGCTGCCTGC CACTGCCCCC TGGAGGCGCC CAAGTGCGCG 121 CCGGGAGTCG GGCTGGTCCG GGACGGCTGC GGCTGCTGTA AGGTCTGCGC CAAGCAGCTC 181 AACGAGGACT GCAGCAAAAC GCAGCCCTGC GACCACACCA AGGGGCTGGA ATGCAACTTC 241 GGCGCCAGCT CCACCGCTCT GAAGGGGATC TGCAGAGCTC AGTCAGAGGG CAGACCCTGT 301 GAATATAACT CCAGAATCTA CCAAAACGGG GAAAGTTTCC AGCCCAACTG TAAACATCAG 361 TGCACATGTA TTGATGGCGC CGTGGGCTGC ATTCCTCTGT GTCCCCAAGA ACTATCTCTC 421 CCCAACTTGG GCTGTCCCAA CCCTCGGCTG GTCAAAGTTA CCGGGCAGTG CTGCGAGGAG 481 TGGGTCTGTG ACGAGGATAG TATCAAGGAC CCCATGGAGG ACCAGGACGG CCTCCTTGGC 541 AAGGAGCTGG GATTCGATGC CTCCGAGGTG GAGTTGACGA GAAACAATGA ATTGATTGCA 601 GTTGGAAAAG GCAGCTCACT GAAGCGGCTC CCTGTTTTTG GAATGGAGCC TCGCATCCTA 661 TACAACCCTT TACAAGGCCA GAAATGTATT GTTCAAACAA CTTCATGGTC CCAGTGCTCA 721 AAGACCTGTG GAACTGGTAT CTCCACACGA GTTACCAATG ACAACCCTGA GTGCCGCCTT 781 GTGAAAGAAA CCCGGATTTG TGAGGTGCGG CCTTGCGGAC AGCCAGTGTA CAGCAGCCTG 841 AAAAAGGGCA AGAAATGCAG CAAGACCAAG AAATCCCCCG AACCAGTCAG GTTTACTTAC 901 GCTGGATGTT TGAGTGTGAA GAAATACCGG CCCAAGTACT GCGGTTCCTG CGTGGACGGC 961 CGATGCTGCA CGCCCCAGCT GACCAGGACT GTGAAGATGC GGTTCCGCTG CGAAGATGGG 1021 GAGACATTTT CCAAGAACGT CATGATGATC CAGTCCTGCA AATGCAACTA CAACTGCCCG 1081 CATGCCAATG AAGCAGCGTT TCCCTTCTAC AGGCTGTTCA ATGACATTCA CAAATTTAGG 1141 GACTGCT1GAT TACAAGGATG ACGACGATAA G2TAA
1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag
Quality Control:cDNA sequencing results is available upon request.
