Human CYR61 ORF Expression Clone, FLAG-tagged

CYR61 cDNA Clone Datasheet

CYR61 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001554.4
• cDNA Size: 1146
• Gene Synonym: CCN1;GIG1;IGFBP10
• Source: Human

CYR61 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens cysteine-rich, angiogenic inducer, 61 DNA.
• Vector: pCMV/hygro-FLAG
• FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 816T/C not causing the amino acid variation.

CYR61 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CYR61 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O Briefly centrifuge the tube for 10 seconds.
3. Incubate the tube at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CYR61 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCTCCC GCATCGCCAG GGCGCTCGCC TTAGTCGTCA CCCTTCTCCA CTTGACCAGG
61       CTGGCGCTCT CCACCTGCCC CGCTGCCTGC CACTGCCCCC TGGAGGCGCC CAAGTGCGCG
121      CCGGGAGTCG GGCTGGTCCG GGACGGCTGC GGCTGCTGTA AGGTCTGCGC CAAGCAGCTC
181      AACGAGGACT GCAGCAAAAC GCAGCCCTGC GACCACACCA AGGGGCTGGA ATGCAACTTC
241      GGCGCCAGCT CCACCGCTCT GAAGGGGATC TGCAGAGCTC AGTCAGAGGG CAGACCCTGT
301      GAATATAACT CCAGAATCTA CCAAAACGGG GAAAGTTTCC AGCCCAACTG TAAACATCAG
361      TGCACATGTA TTGATGGCGC CGTGGGCTGC ATTCCTCTGT GTCCCCAAGA ACTATCTCTC
421      CCCAACTTGG GCTGTCCCAA CCCTCGGCTG GTCAAAGTTA CCGGGCAGTG CTGCGAGGAG
481      TGGGTCTGTG ACGAGGATAG TATCAAGGAC CCCATGGAGG ACCAGGACGG CCTCCTTGGC
541      AAGGAGCTGG GATTCGATGC CTCCGAGGTG GAGTTGACGA GAAACAATGA ATTGATTGCA
601      GTTGGAAAAG GCAGCTCACT GAAGCGGCTC CCTGTTTTTG GAATGGAGCC TCGCATCCTA
661      TACAACCCTT TACAAGGCCA GAAATGTATT GTTCAAACAA CTTCATGGTC CCAGTGCTCA
721      AAGACCTGTG GAACTGGTAT CTCCACACGA GTTACCAATG ACAACCCTGA GTGCCGCCTT
781      GTGAAAGAAA CCCGGATTTG TGAGGTGCGG CCTTGCGGAC AGCCAGTGTA CAGCAGCCTG
841      AAAAAGGGCA AGAAATGCAG CAAGACCAAG AAATCCCCCG AACCAGTCAG GTTTACTTAC
901      GCTGGATGTT TGAGTGTGAA GAAATACCGG CCCAAGTACT GCGGTTCCTG CGTGGACGGC
961      CGATGCTGCA CGCCCCAGCT GACCAGGACT GTGAAGATGC GGTTCCGCTG CGAAGATGGG
1021     GAGACATTTT CCAAGAACGT CATGATGATC CAGTCCTGCA AATGCAACTA CAACTGCCCG
1081     CATGCCAATG AAGCAGCGTT TCCCTTCTAC AGGCTGTTCA ATGACATTCA CAAATTTAGG
1141     GACTGCT1GAT TACAAGGATG ACGACGATAA G2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag

 

 

Quality Control:cDNA sequencing results is available upon request.