Human CYP20A1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13437-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CYP20A1 cDNA Clone Datasheet

CYP20A1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC033752
cDNA Size:	1389
Gene Synonym:	CYP-M; CYP20A1
Source:	Human

CYP20A1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens cytochrome P450, family 20, subfamily A, polypepti DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CYP20A1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CYP20A1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CYP20A1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTGGACT TCGCGATCTT CGCCGTTACC TTCTTGCTGG CGTTGGTGGG AGCCGTGCTC
61       TACCTCTATC CGGCTTCCAG ACAAGCTGCA GGAATTCCAG GGATTACTCC AACTGAAGAA
121      AAAGATGGTA ATCTTCCAGA TATTGTGAAT AGTGGAAGTT TGCATGAGTT CCTGGTTAAT
181      TTGCATGAGA GATATGGGCC TGTGGTCTCC TTCTGGTTTG GCAGGCGCCT CGTGGTTAGT
241      TTGGGCACTG TTGATGTACT GAAGCAGCAT ATCAATCCCA ATAAGACATT GGACCCTTTT
301      GAAACCATGC TGAAGTCATT ATTAAGGTAT CAATCTGGTG GTGGCAGTGT GAGTGAAAAC
361      CACATGAGGA AAAAATTGTA TGAAAATGGT GTGACTGATT CTCTGAAGAG TAACTTTGCC
421      CTCCTCCTAA AGCTTTCAGA AGAATTATTA GATAAATGGC TCTCCTACCC AGAGACCCAG
481      CACGTGCCCC TCAGCCAGCA TATGCTTGGT TTTGCTATGA AGTCTGTTAC ACAGATGGTA
541      ATGGGTAGTA CATTTGAAGA TGATCAGGAA GTCATTCGCT TCCAGAAGAA TCATGGCACA
601      GTTTGGTCTG AGATTGGAAA AGGCTTTCTA GATGGGTCAC TTGATAAAAA CATGACTCGG
661      AAAAAACAAT ATGAAGATGC CCTCATGCAA CTGGAGTCTG TTTTAAGGAA CATCATAAAA
721      GAACGAAAAG GAAGGAACTT CAGTCAACAT ATTTTCATTG ACTCCTTAGT ACAAGGGAAC
781      CTTAATGACC AACAGATCCT AGAAGACAGT ATGATATTTT CTCTGGCCAG TTGCATAATA
841      ACTGCAAAAT TGTGTACCTG GGCAATCTGT TTTTTAACCA CCTCTGAAGA AGTTCAAAAA
901      AAATTATATG AAGAGATAAA CCAAGTTTTT GGAAATGGTC CTGTTACTCC AGAGAAAATT
961      GAGCAGCTCA GATATTGTCA GCATGTGCTT TGTGAAACTG TTCGAACTGC CAAACTGACT
1021     CCAGTTTCTG CCCAGTTTCA AGATATTGAA GGAAAAATTG ACCGATTTAT TATTCCTAGA
1081     GAGACCCTCG TCCTTTATGC CCTTGGTGTG GTACTTCAGG ATCCTAATAC TTGGCCATCT
1141     CCACACAAGT TTGATCCAGA TCGGTTTGAT GATGAATTAG TAATGAAAAC TTTTTCCTCA
1201     CTTGGATTCT CAGGCACACA GGAGTGTCCA GAGTTGAGGT TTGCATATAT GGTGACCACA
1261     GTACTTCTTA GTGTATTGGT GAAGAGACTG CACCTACTTT CTGTGGAGGG ACAGGTTATT
1321     GAAACAAAGT ATGAACTGGT AACATCATCA AGGGAAGAAG CTTGGATCAC TGTCTCAAAG
1381     AGATATTAA

Quality Control:cDNA sequencing results is available upon request.