Human CYP17A1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13779-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CYP17A1 cDNA Clone Datasheet

CYP17A1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC063388
cDNA Size:	1527
Gene Synonym:	RP11-753C18.1;CPT7; CYP17; P450C17;S17AH;CYP17A1
Source:	Human

CYP17A1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens cytochrome P450, family 17, subfamily A, polypeptide 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CYP17A1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CYP17A1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CYP17A1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGGGAGC TCGTGGCTCT CTTGCTGCTT ACCCTAGCTT ATTTGTTTTG GCCCAAGAGA
61       AGGTGCCCTG GTGCCAAGTA CCCCAAGAGC CTCCTGTCCC TGCCCCTGGT GGGCAGCCTG
121      CCATTCCTCC CCAGACACGG CCATATGCAT AACAACTTCT TCAAGCTGCA GAAAAAATAT
181      GGCCCCATCT ATTCGGTTCG TATGGGCACC AAGACTACAG TGATTGTCGG CCACCACCAG
241      CTGGCCAAGG AGGTGCTTAT TAAGAAGGGC AAGGACTTCT CTGGGCGGCC TCAAATGGCA
301      ACTCTAGACA TCGCGTCCAA CAACCGTAAG GGTATCGCCT TCGCTGACTC TGGCGCACAC
361      TGGCAGCTGC ATCGAAGGCT GGCGATGGCC ACCTTTGCCC TGTTCAAGGA TGGCGATCAG
421      AAGCTGGAGA AGATCATTTG TCAGGAAATC AGTACATTGT GTGATATGCT GGCCACCCAC
481      AACGGACAGT CCATAGACAT CTCCTTTCCT GTCTTCGTGG CGGTAACCAA TGTCATCTCC
541      TTGATCTGCT TCAATACCTC CTACAAGAAT GGGGACCCTG AGTTGAATGT CATACAGAAT
601      TACAATGAAG GCATCATAGA CAACCTGAGC AAAGACAGCC TGGTGGACCT AGTCCCCTGG
661      TTGAAGATTT TCCCCAACAA AACCCTGGAA AAATTAAAGA GCCATGTTAA AATACGAAAT
721      GATCTGCTGA ATAAAATACT TGAAAATTAC AAGGAGAAAT TCCGGAGTGA CTCTATCACC
781      AACATGCTGG ACACACTGAT GCAAGCCAAG ATGAACTCAG ATAATGGCAA TGCTGGCCCA
841      GATCAAGACT CAGAGCTGCT TTCAGATAAC CACATTCTCA CCACCATAGG GGACATCTTT
901      GGGGCTGGCG TGGAGACCAC CACCTCTGTG GTTAAATGGA CCCTGGCCTT CCTGCTGCAC
961      AATCCTCAGG TGAAGAAGAA GCTCTACGAG GAGATTGACC AGAATGTGGG TTTCAGCCGC
1021     ACACCAACTA TCAGTGACCG TAACCGTCTC CTCCTGCTGG AGGCCACCAT CCGAGAGGTG
1081     CTTCGCCTCA GGCCCGTGGC CCCTATGCTC ATCCCCCACA AGGCCAACGT TGACTCCAGC
1141     ATCGGTGAGT TTGCTGTGGA CAAGGGCACA GAAGTTATCA TCAATCTGTG GGCGCTGCAT
1201     CACAATGAGA AGGAGTGGCA CCAGCCGGAT CAGTTCATGC CTGAGCGTTT CTTGAATCCA
1261     GCGGGGACCC AGCTCATCTC ACCGTCAGTA AGCTATTTGC CCTTCGGAGC AGGACCTCGC
1321     TCCTGTATAG GTGAGATCCT GGCCCGCCAG GAGCTCTTCC TCATCATGGC CTGGCTGCTG
1381     CAGAGGTTCG ACCTGGAGGT GCCAGATGAT GGGCAGCTGC CCTCCCTGGA AGGCATCCCC
1441     AAGGTGGTCT TTCTGATCGA CTCTTTCAAA GTGAAGATCA AGGTGCGCCA GGCCTGGAGG
1501     GAAGCCCAGG CTGAGGGTAG CACCTAA

Quality Control:cDNA sequencing results is available upon request.