CYB5R1 Protein (His Tag)

Cytochrome b5 reductase 1 Protein Datasheet

CYB5R1 Protein Product Information

• Synonym: UNQ3049, PRO9865, B5R.1, B5R1, NQO3A2, humb5R2
• Protein Construction: A DNA sequence encoding the human CYB5R1 (Q9UHQ9) (Leu 29-Tyr 305) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

CYB5R1 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CYB5R1 consisting of 292 amino acids and migrates as an approximately 33.5 kDa band in SDS-PAGE under reducing conditions as predicted.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CYB5R1 protein

CYB5R1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CYB5R1 Protein Description

NADH-cytochrome b5 reductase 1 (CYB5R1) belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. This reductase contains 1 FAD-binding FR-type domain and is involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Cytochrome b5 reductase, a flavoprotein that has an NADH binding site, can provide electrons to p450 monooxygenases through cytochrome b5. Reactions catalyzed by P450 show that the properties of the associated electron transport proteins that support P450-1 activity in mutants are consistent with cytochroe b5: NADH-cytochrome b5 oxidoreductase (cyt b5: cyt b5 reductase). Experiments have indicated that a flavoprotein is involved in the reaction that GA12 synthesis from GA12-aldehyde and consistent with participation of cytochrome b5 reductase which has an FAD prosthetic group. Added cytochrome b5 would enhance coupling of cytochrome b5 reductase to the P450-1 monooxygenase facilitating electron transport, while added FAD would restore bound flavin that may have been lost from the cyt b5 reductase during microsome preparation.

References

1. Troncoso C. et al., 2008, Phytochemistry. 69 (3): 672-83.
2. Qian W. et al., 1998, Biochemistry. 37 (40): 14137-50.
3. Zhu H. et al., 2000, Proc Natl Acad Sci. 96 (26): 14742-7.