Human CYB5D2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13731-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CYB5D2 cDNA Clone Datasheet

CYB5D2 cDNA Clone Product Information

Gene Bank Ref.ID:	BC020263
cDNA Size:	795
Gene Synonym:	MGC32124; CYB5D2
Source:	Human

CYB5D2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens cytochrome b5 domain containing 2 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

CYB5D2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CYB5D2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CYB5D2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTGAGGT GCGGAGGCCG TGGGCTTTTG TTGGGCCTGG CTGTAGCCGC AGCAGCGGTA
61       ATGGCAGCAC GGCTTATGGG CTGGTGGGGT CCCCGCGCTG GCTTTCGCCT TTTCATACCG
121      GAGGAGCTGT CTCGCTACCG CGGCGGCCCA GGGGACCCGG GCCTGTACTT GGCGTTGCTC
181      GGCCGTGTCT ACGATGTGTC CTCCGGCCGG AGGCACTACG AGCCTGGGTC CCACTATAGC
241      GGCTTCGCAG GCCGAGACGC ATCCAGAGCT TTCGTGACCG GGGACTGTTC TGAAGCAGGC
301      CTCGTGGATG ACGTATCCGA CCTGTCAGCC GCTGAGATGC TGACACTTCA CAATTGGCTT
361      TCATTCTATG AGAAGAATTA TGTGTGTGTT GGGAGGGTGA CAGGACGGTT CTACGGAGAG
421      GATGGGCTGC CCACCCCGGC ACTGACCCAG GTAGAAGCTG CGATCACCAG AGGCTTGGAG
481      GCCAACAAAC TACAGCTGCA AGAGAAGCAG ACATTCCCGC CGTGCAACGC GGAGTGGAGC
541      TCAGCCAGGG GCAGCCGGCT CTGGTGCTCC CAGAAGAGTG GAGGTGTGAG CAGAGACTGG
601      ATTGGCGTCC CCAGGAAGCT GTATAAGCCA GGTGCTAAGG AGCCCCGCTG CGTGTGTGTG
661      AGAACCACCG GCCCCCCTAG TGGCCAGATG CCGGACAACC CTCCACACAG AAATCGTGGG
721      GACCTGGACC ACCCAAACTT GGCAGAGTAC ACAGGCTGCC CACCGCTAGC CATCACATGC
781      TCCTTTCCAC TCTAA

Quality Control:cDNA sequencing results is available upon request.