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Rat CXCR4 Gene ORF cDNA clone in cloning vector

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    Rat CXCR4 cDNA Clone Product Information
    NCBI RefSeq:NM_022205.3
    RefSeq ORF Size:1050bp
    cDNA Description:Full length Clone DNA of Rattus norvegicus chemokine (C-X-C motif) receptor 4.
    Gene Synonym:MGC108696, Cxcr4
    Species:Rat
    Vector:pGEM-T Vector
    Plasmid:pGEM-ratCXCR4
    Restriction Site:
    Tag Sequence:
    Sequence Description:Identical with the Gene Bank Ref. ID sequence.
    Sequencing primers:SP6 and T7 or M13-47 and RV-M
    ( We provide with CXCR4 qPCR primers for gene expression analysis, RP300312 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Storage:The lyophilized plasmid can be stored at room temperature for three months.
    Immunochemical staining of human CCNF in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
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    Catalog: RG80342-G
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