Human CXCL9 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10888-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_002416.1

cDNA Size:   378

Synonym:  CMK; MIG; Humig; SCYB9; crg-10; CXCL9

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens chemokine (C-X-C motif) ligand 9 DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGAAGAAAA GTGGTGTTCT TTTCCTCTTG GGCATCATCT TGCTGGTTCT GATTGGAGTG
61       CAAGGAACCC CAGTAGTGAG AAAGGGTCGC TGTTCCTGCA TCAGCACCAA CCAAGGGACT
121      ATCCACCTAC AATCCTTGAA AGACCTTAAA CAATTTGCCC CAAGCCCTTC CTGCGAGAAA
181      ATTGAAATCA TTGCTACACT GAAGAATGGA GTTCAAACAT GTCTAAACCC AGATTCAGCA
241      GATGTGAAGG AACTGATTAA AAAGTGGGAG AAACAGGTCA GCCAAAAGAA AAAGCAAAAG
301      AATGGGAAAA AACATCAAAA AAAGAAAGTT CTGAAAGTTC GAAAATCTCA ACGTTCTCGT
361      CAAAAGAAGA CTACATAA

Quality Control: cDNA sequencing results is available upon request.

CXCL9/MIG related areas, pathways, and other information

Cytokine

Chemokine