Rat CXCL16 / SRPSOX Protein (His Tag) (Cytokine)

Chemokine (C-X-C motif) ligand 16 Protein Datasheet

CXCL16 / SRPSOX Protein Product Information

• Synonym: CXCL16
• Protein Construction: A DNA sequence encoding the rat CXCL16 (Q6AXU5) extracellular domain (Met 1-Ala 198) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CXCL16 / SRPSOX Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Asn 27
• Molecular Mass: The recombinant rat CXCL16 comprises 183 amino acids and predicts a molecular mass of 20.4 kDa. The apparent molecular mass of the rat CXCL16 is approximately 42 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CXCL16 / SRPSOX protein

CXCL16 / SRPSOX Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CXCL16 / SRPSOX Protein Description

C-X-C motif chemokine 16, also known as Small-inducible cytokine B16, SR-PSOX, and CXCL16, is a single-pass type I membrane protein which belongs to the intercrine alpha (chemokine CxC) family. It is expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. It is weakly expressed in heart and liver and no expression in brain and bone marrow. CXCL16 is expressed on the surface of APCs including subsets of CD19(+) B cells and CD14(+) monocyte / macrophages, and functional CXCL16 is also shed from macrophages. CXCL16 is an alpha (CXC) chemokine but also has characteristics of CC chemokines and a structure similar to fractalkine (neurotactin) in having a transmembrane region and a chemokine domain suspended by a mucin-like stalk. CXCL16 may function in promoting interactions between DCs and CD8 T cells and in guiding T cell movements in the splenic red pulp. CXCL16 was also found in the thymic medulla and in some nonlymphoid tissues, indicating roles in thymocyte development and effector T cell trafficking.

References

1. Matloubian M. et al., 2000, Nat Immunol. 1 (4): 298-304.
2. Wilbanks A. et al., 2001, J Immunol. 166 (8): 5145-54.
3. Abel S. et al., 2004, J Immunol. 172 (10): 6362-72.