CXCL12 / SDF-1 (isoform a) Protein (His Tag) (Cytokine)

Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) Protein Datasheet

CXCL12 / SDF-1 Protein Product Information

• Synonym: CXCL12，PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1
• Protein Construction: A DNA sequence encoding the human CXCL12 isoform alpha (P48061-2) (Lys 22-Lys 89) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

CXCL12 / SDF-1 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CXCL12 consists of 84 amino acids and predicts a molecular mass of 10 KDa. It migrates as an approximately 12KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS，pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CXCL12 / SDF-1 protein

CXCL12 / SDF-1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CXCL12 / SDF-1 Protein Description

Stromal cell-derived factor 1, also known as C-X-C motif chemokine 12, Intercrine reduced in hepatomas, Pre-B cell growth-stimulating factor, SDF-1, PBSF, CXCL12 and SDF1, is a secreted protein which belongs to the intercrine alpha (chemokine CxC) family. CXCL12 / SDF-1 is widely expressed in various organs including heart, liver, brain, kidney, skeletal muscle, and lymphoid organs, and is highly conserved between species. CXCL12 / SDF-1 is expressed in two isoforms from a single gene that encodes two splice variants, SDF1α and SDF1β, which are identical except for the four residues present in the C-terminus of SDF1β but absent from SDF1α. As a highly efficacious lymphocyte chemoattractant, CXCL12 / SDF-1 activates and directs the migration of leukocytes in response to proinflammatory stimuli such as LPS, TNF, or interleukins. It has been demonstrated that CXCL12 / SDF-1 promotes tumor growth and malignancy, enhances tumor angiogenesis, participates in tumor metastasis, and thus plays an important role in carcinoma pathogenesis. In addition, CXCL12 / SDF-1 is identified as the biological ligand for CXC-chemokine receptor 4 (CXCR4), which is widely expressed in leukocytes and acts as the cofactor for HIV-1 entry, and inhibits the HIV-1 infection. Furthermore, SDF1 is rapidly inactivated via N-terminal processing by cathepsin G associated with the membrane of B-cells, NK cells and to a lesser extent, T-cells.

References

1. Bleul CC. et al., 1996, Nature. 382: 829-33.
2. Sapede D. et al., 2005, Proc Natl Acad Sci. 102: 1714-8.
3. Delgado MB. et al., 2001, Eur J Immunol. 31: 699-707.
4. Orimo A. et al., 2005, Cell. 121: 335-48.
5. Kryczek I. et al., 2007, Am J Physiol Cell Physiol. 292: C987-95.
6. Bbalabanian K. et al., 2005, J Biol Chem. 280: 35760-6.