CXCL10 / Crg-2 Protein (Native) (Cytokine)

Chemokine (C-X-C motif) ligand 10 Protein Datasheet

CXCL10 / Crg-2 Protein Product Information

• Synonym: CXCL10, INP10, SCYB10
• Protein Construction: A DNA sequence encoding the mature form of human CXCL10 (Q9UBH0) (Val22-Pro98) was expressed, with a N-terminal Met.
• Source: Human
• Expression Host: E.coli

CXCL10 / Crg-2 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human CXCL10 consists of 78 amino acids and predicts a molecular mass of 8.8 KDa. It migrates as an approximately 9 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CXCL10 / Crg-2 protein

CXCL10 / Crg-2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CXCL10 / Crg-2 Protein Description

CXCL10, also known as crg-2, belongs to the intercrine alpha (chemokine CxC) family. CXC chemokines are particularly significant for leukocyte infiltration in inflammatory diseases. With a three-dimensional crystal structure, CXCL10’s signaling is mediated by the g protein-coupled receptor CXCR3, which is expressed on activated T cells and plays an important role in directing the migration of T cells, especially during Th1 responses. It is secreted by monocytes, epithelial cells, and endothelial cells in response to IFN gamma or other pro-inflammatory cytokines and stimuli. Binding of CXCL10 to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. It is chemotactic for monocytes and T-lymphocytes. Baseline pre-treatment plasma levels of CXCL10 are elevated in patients chronically infected with hepatitis C virus (HCV) of genotypes 1 or 4.

References

1. O'Donovan N. et al., 1999, Cell Genet. 84 (1-2): 39-42.
2. Swaminathan GJ. et al., 2003, Structure. 11 (5): 521-32.
3. Booth V. et al., 2002, Biochemistry. 41 (33): 10418-25.