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Human CXCL1 Gene cDNA Clone (full-length ORF Clone)

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CXCL1cDNA Clone Product Information
Gene Bank Ref.ID:NM_001511.1
cDNA Size:324
cDNA Description:ORF Clone of Homo sapiens chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) DNA.
Gene Synonym:FSP, GRO1, GROa, MGSA, NAP-3, SCYB1, MGSA-a, CXCL1
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping Carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Chemokine & Receptor Related Products
Product nameProduct name
Human CXCL2 / MIP-2 ProteinRat CXCL1 / MGSA / NAP-3 ProteinRat CXCL2 / MIP-2 ProteinHuman CCL2 / MCP-1 / MCP1 Protein (His Tag)Human CCL23 / MIP 3 Protein (His Tag)Mouse NAP-2 / PPBP / CXCL7 Protein (aa 49-109, His Tag)Mouse NAP-2 / PPBP / CXCL7 Protein (aa 40-113, His Tag)Mouse CCL22 / MDC Protein (His Tag)Mouse CXCL14 / BRAK ProteinMouse CXCL1 / MGSA / NAP-3 ProteinHuman NAP-2 / PPBP / CXCL7 Protein (His Tag)Human IL-8 / CXCL8 Protein (aa 28-99)Human CCL13 / MCP-4 Protein (His Tag)Human CCL18 / PARC / MIP4 Protein (His Tag)Mouse CCL2 / MCP-1 / MCP1 Protein (His Tag)Human CCL16 / HCC-4 / NCC4 Protein (His Tag)Human CCL16 / HCC-4 / NCC4 Protein (His Tag)Human CCL4 / MIP1B Protein (His Tag)Human CCL3 / Mip1a Protein (His Tag)Human I-309 / CCL1 / TCA-3 Protein (Fc Tag)Human Fractalkine / CX3CL1 Protein (His Tag)Human CXCL12 / SDF1b Protein (Fc Tag)Human CXCL4 / PF4 ProteinHuman IL-8 / CXCL8 Protein (aa 23-99, Fc Tag)Human IL-8 / CXCL8 Protein (aa 23-99)Human IL-8 / CXCL8 Protein (aa 28-99, Fc Tag)Human IL-8 / CXCL8 Protein (aa 28-99)Human CXCL3 / GRO gamma Protein (His Tag)Human CCL5 / RANTES Protein (His & mucin Tag)Mouse CXCL16 / SR-PSOX Protein (His Tag)Human MCP-3 / CCL7 Protein (His Tag)Human CCL8 / MCP-2 Protein (SUMO Tag)Human CXCL1 / MGSA / NAP-3 Protein (His & SUMO Tag)Human CCL17 / TARC / SCYA17 Protein (His Tag)Mouse CXCL2 / GRO2 / MIP-2 (His & SUMO Tag)Human CCL15 / MIP-5 / MIP-1 delta Protein (aa 22-113, His Tag)Human CCL17 / TARC / SCYA17 ProteinMouse CCL8 / MCP-2 Protein (His & NusA Tag)Mouse CCL6 / C-C motif ligand 6 Protein (His Tag)Cynomolgus CXCL13 / BCA-1 / BLC Protein (His Tag)Human CXCL1 / MGSA / NAP-3 Protein (His & NusA Tag)Cynomolgus CXCL12 / SDF-1 Protein (Fc Tag)Mouse CXCL9 / MIG / C-X-C motif chemokine 9 ProteinHuman CXCL9 / MIG / C-X-C motif chemokine 9 ProteinHuman CCL21 / 6Ckine ProteinHuman CCL14 / HCC-1 / HCC-3 Protein (His Tag)Mouse CCL17 / TARC ProteinCynomolgus / Rhesus Fractalkine / CX3CL1 Protein (Fc Tag)Human CCL14 / HCC-1 / HCC-3 Protein (aa 28-93, His Tag)Human MCP-3 / CCL7 Protein (His Tag)Mouse I-309 / CCL1 / TCA-3 Protein (Fc Tag)Human CCL11 Protein (His Tag)Human CXCL12 / SDF-1 ProteinMouse XCL1 Protein (His Tag)Rat NAP-2 / PPBP / CXCL7 Protein (Fc Tag)Human XCL1 Protein (His Tag)Human CCL15 / MIP-5 / MIP-1 delta Protein (aa 46-113, His Tag)Human XCL2 Protein (His Tag)Human CXCL12 / SDF-1 Protein (isoform a, His Tag)Rat CXCL16 / SR-PSOX Protein (His Tag)Cynomolgus XCL1 Protein (His Tag)Human CCL22 / MDC Protein (His Tag)Mouse CCL20 / MIP-3 alpha Protein (His Tag)Human CCL24 / Eotaxin-2 / MPIF-2 Protein (His Tag)Human CXCL12 / SDF-1 Protein (isoform a)Cynomolgus CCL21 / 6Ckine ProteinHuman CXCL1 / MGSA / NAP-3 ProteinHuman FAM19A2 Protein (Fc Tag)Human CXCL10 / Crg-2 ProteinCanine IL-8 / CXCL8 ProteinRat CXCL16 / SR-PSOX Protein (Fc Tag)Cynomolgus NAP-2 / PPBP / CXCL7 Protein (Fc Tag)Human CXCL5 ProteinHuman I-309 / CCL1 / TCA-3 ProteinMouse CCL3 / Mip1a ProteinCynomolgus CCL17 / TARC Protein (His Tag)Mouse CXCL12 / SDF-1 ProteinCynomolgus CXCL9 / MIG / C-X-C motif chemokine 9 ProteinCynomolgus IL-8 / CXCL8 ProteinCanine CXCL12 / SDF-1 ProteinCanine CXCL13 / BCA-1 Protein (His Tag)Human CCL28 Protein (His Tag)Canine CXCL16 / SR-PSOX Protein (His Tag)Human CCL27 / CTACK Protein (His Tag)Human CXCL14 / BRAK ProteinHuman CCL23 / MIP 3 Protein (His Tag)Human CCL23 / MIP 3 ProteinCanine CXCL16 / SR-PSOX Protein (Fc Tag)Canine Fractalkine / CX3CL1 Protein (Fc Tag)Canine Fractalkine / CX3CL1 ProteinCanine XCL1 Protein (His Tag)Human CCL20 / MIP-3 alpha Protein (His Tag)Rat CCL3 / Mip1a ProteinHuman FAM19A4 / TAFA4 Protein (Fc Tag)Mouse MCP-3 / CCL7 Protein (His Tag)Human CCL5 / RANTES Protein
Background

The Chemokine (C-X-C motif) Ligand 1, CXCL1, is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GRO?, KC, Neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MSGA-a). CXCL1 already known to be important in osteoarthritis (OA), as a novel target gene of transcription factor AP-2? in chondrocytes and support the important role of AP-2? in cartilage. CXCL1 is a potent neutrophil chemoattractant with recognized roles in angiogenesis and inflammation. CXCL1 is a novel immediate PTH/PTHrP-responsive gene. CXCL1 may act as a chemoattractant for osteoclast precursors. CXCL1 may also have important pro-nociceptive effects via its direct actions on sensory neurons, and may induce long-term changes that involve protein synthesis. CXCL1 plays a critical nonredundant role in the development of experimental Lyme arthritis and carditis via CXCR2-mediated recruitment of neutrophils into the site of infection. CXCL1 functions through CXCR2 to transactivate the EGFR by proteolytic cleavage of HB-EGF, leading to activation of MAPK signalling and increased proliferation of epithelial ovarian cancer (EOC) cells. It might limit tumor growth by reinforcing senescence early in tumorigenesis. Thus, CXCL1 plays a role in spinal cord development by inhibiting the migration of oligodendrocyte precursors and is involved in the processes of angiogenesis, inflammation, wound healing, and tumorigenesis.

References
  • Wang JG, et al. (2008) The chemokine CXCL1/growth related oncogene increases sodium currents and neuronal excitability in small diameter sensory neurons. Mol Pain. 4: 38.
  • Acosta JC, et al. (2009) A role for CXCR2 in senescence, but what about in cancer? Cancer Res. 69(6): 2167-70.
  • Onan D, et al. (2009) The chemokine Cxcl1 is a novel target gene of parathyroid hormone (PTH)/PTH-related protein in committed osteoblasts. Endocrinology. 150(5): 2244-53.
  • Ritzman AM, et al. (2010) The chemokine receptor CXCR2 ligand KC (CXCL1) mediates neutrophil recruitment and is critical for development of experimental Lyme arthritis and carditis. Infect Immun. 78(11): 4593-600.
  • Bolitho C, et al. (2010) The chemokine CXCL1 induces proliferation in epithelial ovarian cancer cells by transactivation of the epidermal growth factor receptor. Endocr Relat Cancer. 17(4): 929-40.
  • Wenke AK, et al. (2011) The transcription factor AP-2? regulates CXCL1 during cartilage development and in osteoarthritis. Osteoarthritis Cartilage. 19(2): 206-12.
  • Catalog:HG10877-M
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