CXADR Antibody (Antigen Affinity Purified)

CXADR Antibody ( Antigen Affinity Purified ) Datasheet

CXADR Antibody Product Information

• Immunogen: Recombinant Mouse CXADR protein (Catalog#50019-M08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CXADR (rM CXADR; Catalog#50019-M08H; NP_001020363.1; Met 1-Gly 237). CXADR specific IgG was purified by Mouse CXADR affinity chromatography.

CXADR Antibody Usage Guide

• Specificity: Mouse CXADR
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of mouse CXADR in mouse liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunochemical staining of mouse CXADR in mouse stomach with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CXADR. The detection limit for Mouse CXADR is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CXADR Antibody Background

CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a 46 kDa type I transmembrane glycoprotein expressed on the basolateralsurface, and belongs to the CTX family of the Ig superfamily. It is a receptor for group B coxsackieviruses and subgroup C adenoviruses and also acts as a homophilic adhesion molecule within junctional complexes, notably between epithelial cells lining body cavities and within myocardial intercalated discs. CXADR structrually comprises two extracellular immunoglobulin domains, a transmembrane helix and a C-terminal intracellular domain. The N-terminal Ig domain (D1) of CXADR is necessary and sufficient for adenovirus binding. The C-terminus interacts with several cytoplasmic junctional proteins, microtubules and the actin cytoskeleton. Pairs of transmembrane domains of CXADR associate with each other in a detergent cloud that mimics a cellular plasma membrane. Variants of CXADR areattached to the cell membrane by a GPI-anchor.

References

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