Mouse CTNNB1 / Catenin beta-1 Protein (GST Tag)

Catenin (cadherin associated protein), beta 1 Protein Datasheet

CTNNB1 / Catenin beta-1 Protein Product Information

• Synonym: CTNNB1, Catnb
• Protein Construction: A DNA sequence encoding the mouse CTNNB1 (Q02248) (Met1-Leu781) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

CTNNB1 / Catenin beta-1 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse CTNNB1/GST chimera consists of 1018 amino acids and has a calculated molecular mass of 113 kDa. The recombinant protein migrates as an approximately 115 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl, pH 8.0, 10%gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CTNNB1 / Catenin beta-1 protein

CTNNB1 / Catenin beta-1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CTNNB1 / Catenin beta-1 Protein Description

Catenin beta-1, also known as CTNNB1, is a cytoplasm and nucleus protein which belongs to the beta-catenin family. Catenin beta-1 / CTNNB1 contains 12 ARM repeats. Catenin beta-1 / CTNNB1 is expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Catenin beta-1 / CTNNB1 is also expressed in colon. Catenin beta-1 / CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Catenin beta-1 / CTNNB1 is involved in signal transduction through the Wnt pathway. Nuclear beta-catenin is involved in transcriptional regulation by association with transcription factors of the TCF / LEF family. Defects in CTNNB1 are associated with colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB) susceptibility to ovarian cancer (OC).

References

1. Huelsken J.?et al., 1994, J Cell Biol. 127: 2061-9.
2. Butz S. et al., 1994, FEBS Lett. 355: 195-200.
3. Chan E.F. et al., 1999, Nat Genet. 21: 410-3.
4. Kim J-S. et al., 2002, Cancer Res. 62: 2744-8.
5. Van Noort M. et al., 2002,Exp Cell Res. 276: 264-72.