Mouse CTLA4 Gene cDNA Clone / ORF Clone

CTLA4 cDNA Clone Datasheet

CTLA4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009843.3
• cDNA Size: 672
• Gene Synonym: Cd152; Ly-56; Ctla-4; Ctla4
• Source: Mouse

CTLA4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cytotoxic T-lymphocyte-associated protein 4 DNA.
• Vector: Cloning Vector: pMD18-T Simple Vector.

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 186A/G not causing the amino acid variation.

CTLA4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CTLA4 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CTLA4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTTGTC TTGGACTCCG GAGGTACAAA GCTCAACTGC AGCTGCCTTC TAGGACTTGG
61       CCTTTTGTAG CCCTGCTCAC TCTTCTTTTC ATCCCAGTCT TCTCTGAAGC CATACAGGTG
121      ACCCAACCTT CAGTGGTGTT GGCTAGCAGC CATGGTGTCG CCAGCTTTCC ATGTGAATAT
181      TCACCGTCAC ACAACACTGA TGAGGTCCGG GTGACTGTGC TGCGGCAGAC AAATGACCAA
241      ATGACTGAGG TCTGTGCCAC GACATTCACA GAGAAGAATA CAGTGGGCTT CCTAGATTAC
301      CCCTTCTGCA GTGGTACCTT TAATGAAAGC AGAGTGAACC TCACCATCCA AGGACTGAGA
361      GCTGTTGACA CGGGACTGTA CCTCTGCAAG GTGGAACTCA TGTACCCACC GCCATACTTT
421      GTGGGCATGG GCAACGGGAC GCAGATTTAT GTCATTGATC CAGAACCATG CCCGGATTCT
481      GACTTCCTCC TTTGGATCCT TGTCGCAGTT AGCTTGGGGT TGTTTTTTTA CAGTTTCCTG
541      GTCACTGCTG TTTCTTTGAG CAAGATGCTA AAGAAAAGAA GTCCTCTTAC AACAGGGGTC
601      TATGTGAAAA TGCCCCCAAC AGAGCCAGAA TGTGAAAAGC AATTTCAGCC TTATTTTATT
661      CCCATCAACT GA

 

Quality Control:cDNA sequencing results is available upon request.