Mouse CTHRC1 Gene cDNA Clone / ORF Clone

CTHRC1 cDNA Clone Datasheet

CTHRC1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_026778.2
• cDNA Size: 738
• Gene Synonym: 1110014B07Rik; Cthrc1
• Source: Mouse

CTHRC1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus collagen triple helix repeat containing 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CTHRC1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CTHRC1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CTHRC1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCACCCCC AAGGCCGCGC GGCCCCCCCG CAGCTGCTGC TCGGTCTCTT CCTTGTGCTG
61       CTGCTGCTGC TACAGTTGTC CGCACCGATC AGCGCCTCTG AGAACCCCAA GGTGAAGCAA
121      AAAGCGCTGA TCCGGCAGAG GGAGGTGGTA GACCTGTATA ATGGAATGTG TCTACAAGGA
181      CCAGCAGGAG TTCCCGGTCG TGATGGGAGC CCTGGGGCCA ACGGCATTCC TGGCACACCT
241      GGCATCCCAG GTCGGGATGG ATTCAAAGGG GAAAAGGGAG AATGCTTAAG GGAAAGCTTT
301      GAGGAGTCCT GGACCCCAAA CTATAAGCAG TGTTCGTGGA GTTCGCTGAA CTATGGCATA
361      GATCTTGGGA AAATTGCGGA GTGTACATTC ACGAAGATGC GCTCCAACAG TGCTCTGCGA
421      GTTCTGTTCA GTGGCTCGCT TCGGCTCAAA TGCAGGAATG CATGCTGTCA GCGCTGGTAT
481      TTTACATTTA ATGGAGCTGA ATGTTCAGGA CCTCTTCCCA TCGAAGCCAT CATCTATCTG
541      GACCAAGGAA GCCCTGAGTT AAATTCAACT ATTAATATTC ATCGTACTTC CTCTGTGGAA
601      GGACTCTGTG AAGGGATTGG TGCTGGATTG GTAGATGTGG CCATCTGGGT CGGCACCTGT
661      TCAGATTACC CCAAAGGAGA CGCTTCTACT GGATGGAATT CCGTGTCTCG CATCATCATT
721      GAAGAACTAC CGAAATAA

 

Quality Control:cDNA sequencing results is available upon request.