Human CTGF Gene cDNA Clone / ORF Clone

CTGF cDNA Clone Datasheet

CTGF cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001901.2
• cDNA Size: 1050
• Gene Synonym: CCN2;NOV2;HCS24;IGFBP8
• Source: Human

CTGF cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens connective tissue growth factor DNA.
• Vector: Cloning Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CTGF cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CTGF cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CTGF cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACCGCCG CCAGTATGGG CCCCGTCCGC GTCGCCTTCG TGGTCCTCCT CGCCCTCTGC
61       AGCCGGCCGG CCGTCGGCCA GAACTGCAGC GGGCCGTGCC GGTGCCCGGA CGAGCCGGCG
121      CCGCGCTGCC CGGCGGGCGT GAGCCTCGTG CTGGACGGCT GCGGCTGCTG CCGCGTCTGC
181      GCCAAGCAGC TGGGCGAGCT GTGCACCGAG CGCGACCCCT GCGACCCGCA CAAGGGCCTC
241      TTCTGTGACT TCGGCTCCCC GGCCAACCGC AAGATCGGCG TGTGCACCGC CAAAGATGGT
301      GCTCCCTGCA TCTTCGGTGG TACGGTGTAC CGCAGCGGAG AGTCCTTCCA GAGCAGCTGC
361      AAGTACCAGT GCACGTGCCT GGACGGGGCG GTGGGCTGCA TGCCCCTGTG CAGCATGGAC
421      GTTCGTCTGC CCAGCCCTGA CTGCCCCTTC CCGAGGAGGG TCAAGCTGCC CGGGAAATGC
481      TGCGAGGAGT GGGTGTGTGA CGAGCCCAAG GACCAAACCG TGGTTGGGCC TGCCCTCGCG
541      GCTTACCGAC TGGAAGACAC GTTTGGCCCA GACCCAACTA TGATTAGAGC CAACTGCCTG
601      GTCCAGACCA CAGAGTGGAG CGCCTGTTCC AAGACCTGTG GGATGGGCAT CTCCACCCGG
661      GTTACCAATG ACAACGCCTC CTGCAGGCTA GAGAAGCAGA GCCGCCTGTG CATGGTCAGG
721      CCTTGCGAAG CTGACCTGGA AGAGAACATT AAGAAGGGCA AAAAGTGCAT CCGTACTCCC
781      AAAATCTCCA AGCCTATCAA GTTTGAGCTT TCTGGCTGCA CCAGCATGAA GACATACCGA
841      GCTAAATTCT GTGGAGTATG TACCGACGGC CGATGCTGCA CCCCCCACAG AACCACCACC
901      CTGCCGGTGG AGTTCAAGTG CCCTGACGGC GAGGTCATGA AGAAGAACAT GATGTTCATC
961      AAGACCTGTG CCTGCCATTA CAACTGTCCC GGAGACAATG ACATCTTTGA ATCGCTGTAC
1021     TACAGGAAGA TGTACGGAGA CATGGCATGA

 

Quality Control:cDNA sequencing results is available upon request.