CST9L / Testatin Protein

Cystatin 9-like Protein Datasheet

CST9L / Testatin Protein Product Information

• Synonym: UNQ1835, PRO3543, bA218C14.1
• Protein Construction: A DNA sequence encoding the human CST9L (Q9H4G1) (Met 1-His 147)was fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Human
• Expression Host: Human Cells

CST9L / Testatin Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Trp 29
• Molecular Mass: The recombinant human CST9L/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 360 amino acids and has a calculated molecular mass of 41.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCST9L/Fc monomer is approximately 48 kDa.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CST9L / Testatin protein

CST9L / Testatin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CST9L / Testatin Protein Description

Testatin is a member of the Cystatin family. Cystatins comprise genes that all show expression patterns that are strikingly restricted to reproductive tissue. Testatin shows homology to family 2 cystatins, a group of broadly expressed small secretory proteins that are inhibitors of cysteine proteases in vitro but whose in vivo functions are unclear. It is expressed in germ cells and somatic cells in reproductive tissues. The testatin gene was previously isolated in a screen focused on finding novel signaling molecules involved in sex determination and differentiation. testatin is specifically upregulated in pre-Sertoli cells in early fetal development, immediately after the onset of Sry expression, and was therefore considered a strong candidate for involvement in early testis development. Testatin expression is maintained in the adult Sertoli cell, and it can also be found in a small population of germ cells.

References

1. Dickinson DP. et al., 1993, Crit Rev Oral Biol. 4 (3-4): 573-80.
2. Dickinson DP. et al., 1995, Genomics. 24 (1): 172-5.
3. Thiesse M. et al., 1994, DNA Cell Biol. 13 (2): 97-116.