Mouse CSNK2A1 / CK2A1 Protein (GST Tag)

Casein kinase 2, alpha 1 polypeptide Protein Datasheet

CSNK2A1 / CK2A1 Protein Product Information

• Synonym: Csnk2a1, Ckiia
• Protein Construction: A DNA sequence encoding the mouse CSNK2A1 (Q60737) (Met1-Gln391) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

CSNK2A1 / CK2A1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The secreted recombinant mouse CSNK2A1/GST chimera consists of 628 amino acids and has a calculated molecular mass of 72.9 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl, pH 8.0, 10% gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CSNK2A1 / CK2A1 protein

CSNK2A1 / CK2A1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CSNK2A1 / CK2A1 Protein Description

Casein kinase II subunit alpha, also known as CK II alpha, CSNK2A1 and CK2A1, is a member of the protein kinase superfamily, Ser/Thr protein kinase family and CK2 subfamily. Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. This kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. Three transcript variants encoding two different proteins have been found. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains of CSNK2A1 contain the catalytic site. It participates in Wnt signaling. CSNK2A1 is a tetramer composed of an alpha chain, an alpha' and two beta chains. It is also a component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. It interacts with RNPS1 as well.

References

1. Keller DM. et al., 2001, Mol Cell. 7: 283-92.
2. Sayed M. et al., 2000, J Biol Chem. 275 (22): 16569-73.
3. Kristensen. et al., 2004, FEBS Lett (Netherlands). 569 (1-3): 217-23.
4. Trembley JH. et al., 2005, Mol Cell Biol. 25:1446-57.
5. Adachi A. et al., 2009, Genes Cells. 14 (3): 355-69.